IVD Protein, Human (sf9, His)
IVD is a key enzyme responsible for catalyzing the conversion of isovaleryl-CoA/3-methylbutyryl-CoA into 3-methylbut-2-enoyl-CoA, which is an important intermediate step in the leucine catabolism pathway. . This enzyme activity is essential for the breakdown of the branched-chain amino acid leucine and contributes to the regulation of metabolic processes. IVD Protein, Human (sf9, His) is the recombinant human-derived IVD protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
IVD is a key enzyme responsible for catalyzing the conversion of isovaleryl-CoA/3-methylbutyryl-CoA into 3-methylbut-2-enoyl-CoA, which is an important intermediate step in the leucine catabolism pathway. . This enzyme activity is essential for the breakdown of the branched-chain amino acid leucine and contributes to the regulation of metabolic processes. IVD Protein, Human (sf9, His) is the recombinant human-derived IVD protein, expressed by Sf9 insect cells , with N-His labeled tag.
Isovaleryl-CoA dehydrogenase (IVD) is a crucial enzyme in the leucine catabolic pathway, catalyzing the conversion of isovaleryl-CoA/3-methylbutanoyl-CoA to 3-methylbut-2-enoyl-CoA. This enzymatic step is a key intermediate in the breakdown of leucine. In addition to its primary role in leucine metabolism, IVD exhibits the ability to oxidize other saturated short-chain acyl-CoA thioesters, including pentanoyl-CoA, hexenoyl-CoA, and butenoyl-CoA, albeit to a lesser extent. These broader substrate capabilities suggest that IVD may contribute to the oxidation of various short-chain fatty acids, expanding its metabolic role beyond leucine catabolism. The multifunctional nature of IVD underscores its importance in maintaining metabolic homeostasis by participating in the degradation of diverse acyl-CoA substrates.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His
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Accession
P26440-1 (H33-H426)
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Molecular Construction
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N-term
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His
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IVD (H33-H426)
Accession # P26440-1 -
C-term
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Protein Length
Full Length of Isoform-1 Mature Protein
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Synonyms
IVD; Isovaleryl Coenzyme A Dehydrogenase; Isovaleryl-CoA Dehydrogenase; Epididymis Secretory Sperm Binding Protein; Isovaleryl-CoA Dehydrogenase, Mitochondrial; Isovaleryl-CoA Dehydrogenase Isoform 3; Butyryl-CoA Dehydrogenase; Truncated Isovalery CoA Deh
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AA Sequence
HSLLPVDDAINGLSEEQRQLRQTMAKFLQEHLAPKAQEIDRSNEFKNLREFWKQLGNLGVLGITAPVQYGGSGLGYLEHVLVMEEISRASGAVGLSYGAHSNLCINQLVRNGNEAQKEKYLPKLISGEYIGALAMSEPNAGSDVVSMKLKAEKKGNHYILNGNKFWITNGPDADVLIVYAKTDLAAVPASRGITAFIVEKGMPGFSTSKKLDKLGMRGSNTCELIFEDCKIPAANILGHENKGVYVLMSGLDLERLVLAGGPLGLMQAVLDHTIPYLHVREAFGQKIGHFQLMQGKMADMYTRLMACRQYVYNVAKACDEGHCTAKDCAGVILYSAECATQVALDGIQCFGGNGYINDFPMGRFLRDAKLYEIGAGTSEVRRLVIGRAFNADFH
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Predicted Molecular Mass
45.3 kDa
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Molecular Weight
Approximately 43 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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