1. Recombinant Proteins
  2. CD Antigens Receptor Proteins
  3. Epithelial cell CD Proteins Endothelial cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. JAM-A/CD321 Immunoglobulin-like Cell Adhesion Molecules
  5. Junctional Adhesion Molecule A (JAM-A)
  6. JAM-A/CD321 Protein, Rat (HEK293, His)

JAM-A/CD321 Protein, Rat (HEK293, His)

Cat. No.: HY-P73853
COA Handling Instructions

The JAM-A/CD321 protein is critical for the formation of tight junctions in epithelial cells, participating in early junction development and recruiting PARD3. However, the formation of PARD6-PARD3 complex may hinder PARD3-JAM1 interaction, thus preventing tight junction assembly. JAM-A/CD321 Protein, Rat (HEK293, His) is the recombinant rat-derived JAM-A/CD321 protein, expressed by HEK293 , with C-His labeled tag. The total length of JAM-A/CD321 Protein, Rat (HEK293, His) is 212 a.a., with molecular weight of 25 & 28 kDa, respectively.

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Description

The JAM-A/CD321 protein is critical for the formation of tight junctions in epithelial cells, participating in early junction development and recruiting PARD3. However, the formation of PARD6-PARD3 complex may hinder PARD3-JAM1 interaction, thus preventing tight junction assembly. JAM-A/CD321 Protein, Rat (HEK293, His) is the recombinant rat-derived JAM-A/CD321 protein, expressed by HEK293 , with C-His labeled tag. The total length of JAM-A/CD321 Protein, Rat (HEK293, His) is 212 a.a., with molecular weight of 25 & 28 kDa, respectively.

Background

The JAM-A/CD321 protein plays a crucial role in the formation of tight junctions in epithelial cells. It is involved in the early stages of cell junction development and recruits PARD3. However, the formation of the PARD6-PARD3 complex may hinder the interaction between PARD3 and JAM1, leading to the prevention of tight junction assembly. Moreover, JAM-A/CD321 is involved in regulating the transmigration of monocytes, which is essential for maintaining the integrity of the epithelial barrier. It also acts as a ligand for integrin alpha-L/beta-2, facilitating the transmigration of memory T-cells and neutrophils. Additionally, JAM-A/CD321 interacts with the ninth PDZ domain of MPDZ and the first PDZ domain of PARD3, with the association between PARD3 and PARD6B possibly disrupting this interaction. Furthermore, it interacts with ITGAL (via I-domain).

Biological Activity

Measured by the ability of the immobilized protein to inhibit the adhesion of Vitronectin on HUVEC cells. The ED50 for this effect is 0.1574 μg/mL in the presence of 10 ng/mL Vitronectin. Corresponding to a specific activity is 6353.24 U/mg.

Species

Rat

Source

HEK293

Tag

C-6*His

Accession

Q9JHY1 (K27-G238)

Gene ID

116479  [NCBI]

Molecular Construction
N-term
JAM-A (K27-G238)
Accession # Q9JHY1
His
C-term
Synonyms
Junctional Adhesion Molecule A; JAM-A; JAM-1; PAM-1; CD321; F11R; JCAM
AA Sequence

KGSVYSPQTAVQVPENDSVKLPCIYSGFSSPRVEWKFVQGSTTALVCYNNQITVPYADRVTFSSSGITFSSVTRKDNGEYTCMVSEDGGQNYGEVSIHLTVLVPPSKPTVSIPSSVTIGNRAVLTCSEHDGSPPSEYSWFKDGVPMLTADAKKTRAFINSSYTIDPKSGDLVFDPVSAFDSGEYYCEAQNGYGTAMRSEAVRMEAVELNVGG

Molecular Weight

Approximately 25&28 kDa due to the glycosylation

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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JAM-A/CD321 Protein, Rat (HEK293, His)
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HY-P73853
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