KBTBD8 Protein, Human (sf9, His, StrepⅡ)

KBTBD8 in the BCR E3 ubiquitin ligase complex plays a critical regulatory role in neural crest specification. The BCR(KBTBD8) complex coordinates this action by monoubiquitinating NOLC1 and TCOF1. KBTBD8 Protein, Human (sf9, His, Strep) is the recombinant human-derived KBTBD8 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.

KBTBD8 serves as a substrate-specific adapter within the BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex, functioning as a crucial regulator of neural crest specification. The BCR(KBTBD8) complex orchestrates this regulatory role by mediating the monoubiquitination of NOLC1 and TCOF1. Monoubiquitination facilitates the formation of a NOLC1-TCOF1 complex, serving as a platform that connects RNA polymerase I with enzymes responsible for ribosomal processing and modification. This orchestrated interaction leads to the remodeling of the translational program in differentiating cells, favoring neural crest specification. As a component of the BCR(KBTBD8) E3 ubiquitin ligase complex, alongside CUL3 and RBX1, KBTBD8 plays a pivotal role in modulating cellular processes involved in neural crest development.

Human

Sf9 insect cells

N-StrepⅡ;N-8*His

Q8NFY9 (A2-L601)

84541

Partial

AASADLSKSSPTPNGIPSSDPASDAMDPFHACSILKQLKTMYDEGQLTDIVVEVDHGKTFSCHRNVLAAISPYFRSMFTSGLTESTQKEVRIVGVEAESMDLVLNYAYTSRVILTEANVQALFTAASIFQIPSIQDQCAKYMISHLDPQNSIGVFIFADHYGHQELGDRSKEYIRKKFLCVTKEQEFLQLTKDQLISILDSDDLNVDREEHVYESIIRWFEHEQNEREVHLPEIFAKCIRFPLMEDTFIEKIPPQFAQAIAKSCVEKGPSNTNGCTQRLGMTASEMIICFDAAHKHSGKKQTVPCLDIVTGRVFKLCKPPNDLREVGILVSPDNDIYIAGGYRPSSSEVSIDHKAENDFWMYDHSTNRWLSKPSLLRARIGCKLVYCCGKMYAIGGRVYEGDGRNSLKSVECYDSRENCWTTVCAMPVAMEFHNAVEYKEKIYVLQGEFFLFYEPQKDYWGFLTPMTVPRIQGLAAVYKDSIYYIAGTCGNHQRMFTVEAYDIELNKWTRKKDFPCDQSINPYLKLVLFQNKLHLFVRATQVTVEEHVFRTSRKNSLYQYDDIADQWMKVYETPDRLWDLGRHFECAVAKLYPQCLQKVL

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.