KDM1B Protein, Mouse (sf9, GST)
KDM1B Protein, Mouse (sf9, GST) is the recombinant mouse-derived KDM1B, expressed by Sf9 insect cells , with GST labeled tag. ,
- Species: Mouse
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
KDM1B Protein, Mouse (sf9, GST) is the recombinant mouse-derived KDM1B, expressed by Sf9 insect cells , with GST labeled tag. ,
Background
KDM1B is a histone demethylase that specifically removes mono- and di-methyl groups from 'Lys-4' of histone H3 (without affecting tri-methylated state), functioning as a transcriptional corepressor. The enzyme utilizes FAD-dependent oxidation to generate an imine intermediate followed by hydrolysis. During oogenesis, KDM1B is required for de novo DNA methylation of specific imprinted genes. Biochemical studies demonstrate its remarkable selectivity: it shows no activity toward any methylation states of histone H3 'Lys-9', 'Lys-27', 'Lys-36', or histone H4 'Lys-20'.
Technical Parameters
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Species Mouse
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Source Sf9 insect cells
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Tag GST
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Accession
Q8CIG3-1 (M1-F826)
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Gene ID218214
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Protein Length
Full Length of Isoform-1
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Synonyms
KDM1B; BA204B7.3; Prev. AOF1; FLJ33898; Prev. C6orf193; FLJ43328; LSD2; [Histone-H3]-N(6),N(6)-Dimethyl-L-Lysine(4) FAD-Dependent Demethylase; Flavin-Containing Amine Oxidase Domain-Containing Protein 1; Amine Oxidase (Flavin Containing) Domain 1; Lysine-
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AA Sequence
MAASRGRSKKRSNLELSPDNLPLRSSGRQAKKKAVEIPDEDEDGSSEKKYRKCEKAGCTAAYPVCFASASERCAKNGYTSRWYHLSCGEHFCNECFDHYYRSHKDGYDKYSAWKRVWTSNGKTEPSPKAFMADQQLPYWVQCTKPECGKWRQLTKEIQLTPHMARTYRCGMKPNTITKPDTPDHCSFPEDLRVLEVSNHWWYPMLIQPPLLKDSVAAPLLSAYYPDCVGMSPSCTSTHRATVTAATTTTGSASPGEMEPSKAAPSSLVLGMNRYFQPFYQPNECGKALCVRPDVMELDELYEFPEYSRDPTMYLALRNLILALWYTNCKEALTPQKCIPHIIVRGLVRIRCVQEVERILYFMTRKGLINTGVLTVAAGQHLLPKHYHNKSVLVVGAGPAGLAAARQLHNFGMKVTVLEAKDRIGGRVWDDKSFKGVVVGRGPQIVNGCINNPVALMCEQLGISMRKLGERCDLIQEGGRITDPTVDKRMDFHFNALLDVVSEWRKDKTLLQDVPLGEKIEEIYRAFVKESGIQFSELEGQVLQFHLSNLEYACGSSLHQVSARSWDHNEFFAQFAGDHTLLTPGYSTIIEKLAEGLDIRLKSPVQSIDYTGDEVQVTTTDGMGHSAQKVLVTVPLAILQRGAIQFNPPLSEKKMKAINSLGAGIIEKIALQFPYRFWDSKVQGADFFGHVPPSASQRGLFAVFYDMDSQQSVLMSVITGEAVASLRTMDDKQVLQQCMGILRELFKEQEIPEPTKYFVTRWSTEPWIQMAYSFVKTFGSGEAYDIIAEEIQGTVFFAGEATNRHFPQTVTGAYLSGVREASKIAAF
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Molecular Weight
Approximately 118 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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