KDM5A Protein, Human (sf9, Flag)
KDM5A Protein, Human (sf9, Flag) is the recombinant human-derived KDM5A, expressed by Sf9 insect cells , with C-Flag labeled tag. ,
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
KDM5A Protein, Human (sf9, Flag) is the recombinant human-derived KDM5A, expressed by Sf9 insect cells , with C-Flag labeled tag. ,
Background
KDM5A is a histone demethylase that specifically targets histone H3 at 'Lys-4', playing a pivotal role in histone code regulation. It does not act on H3 'Lys-9', 'Lys-27', 'Lys-36', 'Lys-79' or H4 'Lys-20', and only demethylates trimethylated/dimethylated (not monomethylated) H3 'Lys-4'. It regulates gene transcription by binding to 5'-CCGCCC-3' DNA motifs, potentially enhancing nuclear receptor-mediated transcription and modulating Hox protein expression during cell differentiation. KDM5A also represses cytokine transcription (e.g., CXCL12) and maintains circadian rhythm periodicity. In a demethylase-independent manner, it coactivates CLOCK-BMAL1-driven transcription of PER1/2 and clock-controlled genes by inhibiting HDAC1 to increase histone acetylation at PER1/2 promoters (By similarity). Evidence suggests it may act as a corepressor for genes like MT1F and promote cancer cell proliferation.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag C-Flag
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Accession
P29375-1 (M1-R1090)
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Gene ID5927
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Molecular Construction
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N-term
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KDM5A (M1-R1090)
Accession # P29375-1 -
Flag
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C-term
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Protein Length
Partial
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Synonyms
KDM5A; Histone Demethylase JARID1A; Prev. RBBP2; RBP2; Prev. JARID1A; Jumonji, AT Rich Interactive Domain 1A (RBBP2-Like); Lysine-Specific Demethylase 5A; Jumonji, AT Rich Interactive Domain 1A (RBP2-Like); [Histone H3]-Trimethyl-L-Lysine(4) Demethylase 5
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AA Sequence
MAGVGPGGYAAEFVPPPECPVFEPSWEEFTDPLSFIGRIRPLAEKTGICKIRPPKDWQPPFACEVKSFRFTPRVQRLNELEAMTRVRLDFLDQLAKFWELQGSTLKIPVVERKILDLYALSKIVASKGGFEMVTKEKKWSKVGSRLGYLPGKGTGSLLKSHYERILYPYELFQSGVSLMGVQMPNLDLKEKVEPEVLSTDTQTSPEPGTRMNILPKRTRRVKTQSESGDVSRNTELKKLQIFGAGPKVVGLAMGTKDKEDEVTRRRKVTNRSDAFNMQMRQRKGTLSVNFVDLYVCMFCGRGNNEDKLLLCDGCDDSYHTFCLIPPLPDVPKGDWRCPKCVAEECSKPREAFGFEQAVREYTLQSFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSSIEEDVIVEYGADISSKDFGSGFPVKDGRRKILPEEEEYALSGWNLNNMPVLEQSVLAHINVDISGMKVPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPSHAAEQLEEVMRELAPELFESQPDLLHQLVTIMNPNVLMEHGVPVYRTNQCAGEFVVTFPRAYHSGFNQGYNFAEAVNFCTADWLPIGRQCVNHYRRLRRHCVFSHEELIFKMAADPECLDVGLAAMVCKELTLMTEEETRLRESVVQMGVLMSEEEVFELVPDDERQCSACRTTCFLSALTCSCNPERLVCLYHPTDLCPCPMQKKCLRYRYPLEDLPSLLYGVKVRAQSYDTWVSRVTEALSANFNHKKDLIELRVMLEDAEDRKYPENDLFRKLRDAVKEAETCASVAQLLLSKKQKHRQSPDSGRTRTKLTVEELKAFVQQLFSLPCVISQARQVKNLLDDVEEFHERAQEAMMDETPDSSKLQMLIDMGSSLYVELPELPRLKQELQQARWLDEVRLTLSDPQQVTLDVMKKLIDSGVGLAPHHAVEKAMAELQELLTVSERWEEKAKVCLQARPRHSVASLESIVNEAKNIPAFLPNVLSLKEALQKAREWTAKVEAIQSGSNYAYLEQLESLSAKGRPIPVRLEALPQVESQVAAARAWRERTGRTFLKKNSSHTLLQVLSPRTDIGVYGSGKNRR
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Predicted Molecular Mass
125 kDa
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Molecular Weight
Approximately 125-130 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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