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Kininogen-1 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

Together with endopeptidase inhibitor, protease, and signaling receptor binding activities, the Kininogen-1 protein is predicted to play a critical role in antimicrobial response, coagulation regulation, and control of endopeptidase activity.Kininogen-1 is located in the collagen-containing matrix and shows biased expression, particularly in the liver at embryonic (E18) and adult stages (RPKM 646.0 and 179.1).Kininogen-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Kininogen-1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Kininogen-1 Protein, Mouse (HEK293, His)
  • Kininogen-1 Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Together with endopeptidase inhibitor, protease, and signaling receptor binding activities, the Kininogen-1 protein is predicted to play a critical role in antimicrobial response, coagulation regulation, and control of endopeptidase activity.Kininogen-1 is located in the collagen-containing matrix and shows biased expression, particularly in the liver at embryonic (E18) and adult stages (RPKM 646.0 and 179.1).Kininogen-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Kininogen-1 protein, expressed by HEK293 , with C-His labeled tag.

Background

Kininogen-1 Protein, with predicted functions including cysteine-type endopeptidase inhibitor activity, protease binding activity, and signaling receptor binding activity, plays a crucial role in various biological processes. Its involvement spans antimicrobial humoral immune responses, negative regulation of blood coagulation, and the negative regulation of endopeptidase activity. Situated in the collagen-containing extracellular matrix, Kininogen-1 demonstrates a biased expression pattern, with notable levels in the liver at both embryonic (E18) and adult stages (RPKM 646.0 and 179.1, respectively), highlighting its significance in hepatic functions across different developmental stages.

Verified Bioactivity

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (HY-P1759B). The IC50 value is 1-3 nM that incubate at 37°C for 15 minutes. (Activation description: The proenzyme needs to be activated in reducing buffer for an activated form.)

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-10*His
  • Accession

    NP_001095882.1 (E21-S480)

  • Gene ID
    16644  [NCBI]
  • Molecular Construction
    • N-term
    • Kininogen-1 (E21-S480)
      Accession # NP_001095882.1
    • 10*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    KNG1; Fitzgerald Factor; Prev. BDK; Bradykinin; Prev. KNG; CDNA FLJ56836, Highly Similar To Kininogen-1; Prev. HK; High-Molecular-Weight Kininogen; HMWK; Kininogen 1, Isoform CRA_b; Alpha-2-Thiol Proteinase Inhibitor; KNG1 Protein; BK; Kininogen; Williams

  • AA Sequence

    EEAQEIDCNDEAVFQAVDFSLKQFNPGVKSGNQYMLHRVIEGTKTDGSPTFYSFKYLIKEGNCSAQSGLAWQDCDFKDAEEAATGECTATVGKRENEFFIVTQTCKIAPSKAPILKAYFPCIGCVHAISTDSPDLEPVLKHSIEHFNNNTDHSHLFTLRKVKSAHRQVVAGLNFDITYTIVQTNCSKERFPSLHGDCVALPNGDDGECRGNLFMDINNKIANFSQSCTLYSGDDLVEALPKPCPGCPRDIPVDSPELKEVLGHSIAQLNAENDHPFYYKIDTVKKATSQVVAGTKYVIEFIARETKCSKESNTELAEDCEIKHLGQSLDCNANVYMRPWENKVVPTVKCQALDMTEMARRPPGFSPFRSVTVQETKEGRTDSDFIEDVVATTPPYDTGAHDDLIPDIHVQPDSLSFKLISDFPEATSPKCPGRPWKPASWKDPNTETTEFSDFDLLDALS

  • Molecular Weight

    Approximately 70-80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 50 mM NaCl, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Kininogen-1 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Kininogen-1 Protein, Mouse (HEK293, His)
  • Kininogen-1 Protein, Mouse (HEK293, His) Product Image