Kininogen-1 Protein, Mouse (HEK293, His)

Together with endopeptidase inhibitor, protease, and signaling receptor binding activities, the Kininogen-1 protein is predicted to play a critical role in antimicrobial response, coagulation regulation, and control of endopeptidase activity. Kininogen-1 is located in the collagen-containing matrix and shows biased expression, particularly in the liver at embryonic (E18) and adult stages (RPKM 646.0 and 179.1). Kininogen-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Kininogen-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Kininogen-1 Protein, Mouse (HEK293, His) is 460 a.a., with molecular weight of 70-95 kDa.

Kininogen-1 Protein, with predicted functions including cysteine-type endopeptidase inhibitor activity, protease binding activity, and signaling receptor binding activity, plays a crucial role in various biological processes. Its involvement spans antimicrobial humoral immune responses, negative regulation of blood coagulation, and the negative regulation of endopeptidase activity. Situated in the collagen-containing extracellular matrix, Kininogen-1 demonstrates a biased expression pattern, with notable levels in the liver at both embryonic (E18) and adult stages (RPKM 646.0 and 179.1, respectively), highlighting its significance in hepatic functions across different developmental stages.

Mouse

HEK293

C-His

NP_001095882.1 (E21-S480)

16644  [NCBI]

EEAQEIDCNDEAVFQAVDFSLKQFNPGVKSGNQYMLHRVIEGTKTDGSPTFYSFKYLIKEGNCSAQSGLAWQDCDFKDAEEAATGECTATVGKRENEFFIVTQTCKIAPSKAPILKAYFPCIGCVHAISTDSPDLEPVLKHSIEHFNNNTDHSHLFTLRKVKSAHRQVVAGLNFDITYTIVQTNCSKERFPSLHGDCVALPNGDDGECRGNLFMDINNKIANFSQSCTLYSGDDLVEALPKPCPGCPRDIPVDSPELKEVLGHSIAQLNAENDHPFYYKIDTVKKATSQVVAGTKYVIEFIARETKCSKESNTELAEDCEIKHLGQSLDCNANVYMRPWENKVVPTVKCQALDMTEMARRPPGFSPFRSVTVQETKEGRTDSDFIEDVVATTPPYDTGAHDDLIPDIHVQPDSLSFKLISDFPEATSPKCPGRPWKPASWKDPNTETTEFSDFDLLDALS

70-95 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 50 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.