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Kirrel1/NEPH1 Protein, Human (HEK293, His)

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The Kirrel1/NEPH1 protein is essential for the normal function of the glomerular filtration barrier in the kidney, maintaining stable podocyte structure through finger-like foot processes and gap septal cell-cell junctions. As a signaling molecule, it requires TEC kinase to fully activate the transcription factor AP-1. Kirrel1/NEPH1 Protein, Human (HEK293, His) is the recombinant human-derived Kirrel1/NEPH1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The Kirrel1/NEPH1 protein is essential for the normal function of the glomerular filtration barrier in the kidney, maintaining stable podocyte structure through finger-like foot processes and gap septal cell-cell junctions. As a signaling molecule, it requires TEC kinase to fully activate the transcription factor AP-1. Kirrel1/NEPH1 Protein, Human (HEK293, His) is the recombinant human-derived Kirrel1/NEPH1 protein, expressed by HEK293 , with C-His labeled tag.

Background

Kirrel1/NEPH1 Protein is crucial for the proper function of the glomerular filtration barrier in the kidneys. It plays a vital role in maintaining a stable podocyte architecture by facilitating the interdigitating foot processes connected by specialized cell-cell junctions called the slit diaphragm. This protein acts as a signaling molecule and requires the presence of TEC kinases to fully activate the transcription factor AP-1. Kirrel1/NEPH1 interacts with TJP1/ZO-1 and NPHS2/podocin, and its interaction with NPHS1/nephrin relies on Kirrel1/NEPH1 glycosylation. It forms homodimers through its Ig-like domains and, when tyrosine-phosphorylated, it interacts with GRB2.

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells. When cells are added to KIRREL coated plates (15 μg/mL, 100 μL/well), > 20% will adhere specifically after 90 minutes at 37 °C.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q96J84-1 (Q17-L493)

  • Gene ID
    55243  [NCBI]
  • Molecular Construction
    • N-term
    • Kirrel1/NEPH1 (Q17-L493)
      Accession # Q96J84-1
    • His
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    KIRREL1; Kin Of Irregular Chiasm-Like Protein 1; Prev. KIRREL; Kin Of IRRE Like (Drosophila); NEPH1; Kin Of IRRE Like; Kin Of IRRE-Like Protein 1; Nephrin Related; Nephrin-Like Protein 1; NPHS23; Kirre Like Nephrin Family Adhesion Molecule 1; CDNA FLJ5636

  • AA Sequence

    QGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVL

  • Predicted Molecular Mass

    53.5 kDa

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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