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Lactoferrin/LTF Protein, Mouse (HEK293, His)

Cat. No.: HY-P74789
COA Handling Instructions

Lactoferrin (LTF) is a major iron-binding protein in exocrine fluid and exhibits multiple antibacterial effects by chelating iron and disrupting bacterial membranes.It prevents biofilm formation, affects bone metabolism, and affects adenovirus and papillomavirus infections.Lactoferrin/LTF Protein, Mouse (HEK293, His) is the recombinant mouse-derived Lactoferrin/LTF protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

Lactoferrin (LTF) is a major iron-binding protein in exocrine fluid and exhibits multiple antibacterial effects by chelating iron and disrupting bacterial membranes.It prevents biofilm formation, affects bone metabolism, and affects adenovirus and papillomavirus infections.Lactoferrin/LTF Protein, Mouse (HEK293, His) is the recombinant mouse-derived Lactoferrin/LTF protein, expressed by HEK293 , with C-His labeled tag.

Background

Lactoferrin (LTF), a major iron-binding and multifunctional protein present in exocrine fluids such as breast milk and mucosal secretions, exhibits diverse biological activities. Renowned for its antimicrobial properties, LTF hinders microbial growth through sequestering free iron and releasing lipopolysaccharides from the bacterial outer membrane, thereby displaying both bacteriostatic and bactericidal effects. Moreover, it plays a role in preventing bacterial biofilm development in Pseudomonas aeruginosa infections and demonstrates weak antifungal activity against Candida albicans. Beyond its antimicrobial functions, LTF impacts bone metabolism by exerting anabolic, differentiating, and anti-apoptotic effects on osteoblasts, while also inhibiting osteoclastogenesis. It contributes to adenovirus infection by promoting the binding of species C adenoviruses to epithelial cells and inhibits papillomavirus infections. LTF stimulates the TLR4 signaling pathway, leading to NF-kappa-B activation and pro-inflammatory cytokine production, while concurrently interfering with lipopolysaccharide-stimulated TLR4 signaling. Additionally, it exhibits inhibitory effects on neutrophil granulocyte migration and promotes VEGFA-mediated endothelial cell migration and proliferation. LTF's versatility extends to its binding capabilities, encompassing interactions with heparin, chondroitin sulfate, pneumococcal surface protein A, lysozyme, and DNA.

Biological Activity

Measured by its ability to increase the expression of TNF-α in THP-1 cells. The ED50 for this effect is 37.15 ng/mL, corresponding to a specific activity is 2.69×104 U/mg.

Species

Mouse

Source

HEK293

Tag

C-His

Accession

AAH06904 (T22-Q707)

Gene ID
Molecular Construction
N-term
LTF (T22-Q707)
Accession # AAH06904
His
C-term
Synonyms
Lactotransferrin; Lactoferrin; Kaliocin-1; Lactoferroxin-A; LTF
AA Sequence

TTVRWCAVSNSEEEKCLRWQNEMRKVGGPPLSCVKKSSTRQCIQAIVTNRADAMTLDGGTMFDAGKPPYKLRPVAAEVYGTKEQPRTHYYAVAVVKNSSNFHLNQLQGLRSCHTGIGRSAGWKIPIGTLRPYLNWNGPPASLEEAVSKFFSKSCVPGAQKDRFPNLCSSCAGTGANKCASSPEEPYSGYAGALRCLRDNAGDVAFTRGSTVFEELPNKAERDQYKLLCPDNTWKPVTEYKECHLAQVPSHAVVSRSTNDKEEAIWELLRQSQEKFGKKQASGFQLFASPSGQKDLLFKESAIGFVRVPQKVDVGLYLTFSYTTSIQNLNKKQQDVIASKARVTWCAVGSEEKRKCDQWNRASRGRVTCISFPTTEDCIVAIMKGDADAMSLDGGYIYTAGKCGLVPVLAENQKSSKSNGLDCVNRPVEGYLAVAAVRREDAGFTWSSLRGKKSCHTAVDRTAGWNIPMGLLANQTRSCKFNEFFSQSCAPGADPKSNLCALCIGDEKGENKCAPNSKERYQGYTGALRCLAEKAGNVAFLKDSTVLQNTDGKNTEEWARNLKLKDFELLCLDDTRKPVTEAKNCHLAIAPNHAVVSRTDKVEVLQQVLLDQQVQFGRNGQRCPGEFCLFQSKTKNLLFNDNTECLAKIPGKTTSEKYLGKEYVIATERLKQCSSSPLLEACAFLTQ

Molecular Weight

Approximately 70 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization) or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Lactoferrin/LTF Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Lactoferrin/LTF Protein, Mouse (HEK293, His)
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HY-P74789
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