Lactoferrin/LTF Protein, Mouse (HEK293, His)

Lactoferrin (LTF) is a major iron-binding protein in exocrine fluid and exhibits multiple antibacterial effects by chelating iron and disrupting bacterial membranes. It prevents biofilm formation, affects bone metabolism, and affects adenovirus and papillomavirus infections. Lactoferrin/LTF Protein, Mouse (HEK293, His) is the recombinant mouse-derived Lactoferrin/LTF protein, expressed by HEK293 , with C-His labeled tag. The total length of Lactoferrin/LTF Protein, Mouse (HEK293, His) is 686 a.a., with molecular weight of ~70 kDa.

Lactoferrin (LTF), a major iron-binding and multifunctional protein present in exocrine fluids such as breast milk and mucosal secretions, exhibits diverse biological activities. Renowned for its antimicrobial properties, LTF hinders microbial growth through sequestering free iron and releasing lipopolysaccharides from the bacterial outer membrane, thereby displaying both bacteriostatic and bactericidal effects. Moreover, it plays a role in preventing bacterial biofilm development in Pseudomonas aeruginosa infections and demonstrates weak antifungal activity against Candida albicans. Beyond its antimicrobial functions, LTF impacts bone metabolism by exerting anabolic, differentiating, and anti-apoptotic effects on osteoblasts, while also inhibiting osteoclastogenesis. It contributes to adenovirus infection by promoting the binding of species C adenoviruses to epithelial cells and inhibits papillomavirus infections. LTF stimulates the TLR4 signaling pathway, leading to NF-kappa-B activation and pro-inflammatory cytokine production, while concurrently interfering with lipopolysaccharide-stimulated TLR4 signaling. Additionally, it exhibits inhibitory effects on neutrophil granulocyte migration and promotes VEGFA-mediated endothelial cell migration and proliferation. LTF's versatility extends to its binding capabilities, encompassing interactions with heparin, chondroitin sulfate, pneumococcal surface protein A, lysozyme, and DNA.

Measured by its ability to increase the expression of TNF-α in THP-1 cells. The ED₅₀ for this effect is 37.15 ng/mL, corresponding to a specific activity is 2.69×10⁴ U/mg.

Mouse

HEK293

C-His

AAH06904 (T22-Q707)

17002  [NCBI]

TTVRWCAVSNSEEEKCLRWQNEMRKVGGPPLSCVKKSSTRQCIQAIVTNRADAMTLDGGTMFDAGKPPYKLRPVAAEVYGTKEQPRTHYYAVAVVKNSSNFHLNQLQGLRSCHTGIGRSAGWKIPIGTLRPYLNWNGPPASLEEAVSKFFSKSCVPGAQKDRFPNLCSSCAGTGANKCASSPEEPYSGYAGALRCLRDNAGDVAFTRGSTVFEELPNKAERDQYKLLCPDNTWKPVTEYKECHLAQVPSHAVVSRSTNDKEEAIWELLRQSQEKFGKKQASGFQLFASPSGQKDLLFKESAIGFVRVPQKVDVGLYLTFSYTTSIQNLNKKQQDVIASKARVTWCAVGSEEKRKCDQWNRASRGRVTCISFPTTEDCIVAIMKGDADAMSLDGGYIYTAGKCGLVPVLAENQKSSKSNGLDCVNRPVEGYLAVAAVRREDAGFTWSSLRGKKSCHTAVDRTAGWNIPMGLLANQTRSCKFNEFFSQSCAPGADPKSNLCALCIGDEKGENKCAPNSKERYQGYTGALRCLAEKAGNVAFLKDSTVLQNTDGKNTEEWARNLKLKDFELLCLDDTRKPVTEAKNCHLAIAPNHAVVSRTDKVEVLQQVLLDQQVQFGRNGQRCPGEFCLFQSKTKNLLFNDNTECLAKIPGKTTSEKYLGKEYVIATERLKQCSSSPLLEACAFLTQ

Approximately 70 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization) or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.