LAMP1/CD107a Protein, Human (HEK293, His)
Based on 1 Customer Validation
LAMP1/CD107a Protein, Human (HEK293, His) is a recombinant human CD107a expressed in HEK 293 cells with a His tag at the N-terminus. LAMP1/CD107a Protein can be used as a functional marker for the identification of natural killer cell activity.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
LAMP1/CD107a Protein, Human (HEK293, His) is a recombinant human CD107a expressed in HEK 293 cells with a His tag at the N-terminus. LAMP1/CD107a Protein can be used as a functional marker for the identification of natural killer cell activity[1][2].
Background
Lysosomal associated membrane protein 1 (LAMP1 or CD107a) is a highly glycosylated lysosomal and endosomal membrane protein of 120 kDa. LAMPs and LIMPs are tightly packed and represent more than 50% of the total membrane protein of late endosomes and lysosomes. LAMP1 has been described as a marker of CD8+ T-cell degranulation following stimulation. LAMP1 can be used as a functional marker for the identification of natural killer cell activity[1][2].
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P11279-1 (A29-M382)
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Molecular Construction
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N-term
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LAMP1 (A29-M382)
Accession # P11279-1 -
6*His
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C-term
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Protein Length
Lumenal Domain
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Synonyms
LAMP1; Lysosomal-Associated Membrane Protein 1; Lysosome Associated Membrane Protein 1; Lysosome-Associated Membrane Protein 1; Lysosomal Associated Membrane Protein 1; CD107a Antigen; CD107a; LGP120; Lysosome-Associated Membrane Glycoprotein 1; LAMP-1; C
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AA Sequence
AMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSM
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Predicted Molecular Mass
39.4 kDa
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Molecular Weight
Approximately 55-120 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Alter G, et, al. CD107a as a functional marker for the identification of natural killer cell activity. J Immunol Methods. 2004 Nov;294(1-2):15-22. [Content Brief]
[2]. Eskelinen EL, et, al. At the acidic edge: emerging functions for lysosomal membrane proteins. Trends Cell Biol. 2003 Mar;13(3):137-45. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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