Lanosterol synthase/LSS Protein, Human (P.pastoris, His)
LSS protein, a pivotal enzyme in cholesterol biosynthesis, catalyzes the cyclization of (S)-2,3 oxidosqualene into lanosterol, a crucial step in sterol nucleus formation. Beyond cholesterol biosynthesis, LSS may regulate lens protein aggregation, maintaining lens transparency. LSS's enzymatic action impacts fundamental and specialized cellular processes, underscoring its significance in diverse physiological contexts. Lanosterol synthase/LSS Protein, Human (P.pastoris, His) is the recombinant human-derived Lanosterol synthase/LSS protein, expressed by P. pastoris , with N-His labeled tag.
- Species: Human
- Source: P. pastoris
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
LSS protein, a pivotal enzyme in cholesterol biosynthesis, catalyzes the cyclization of (S)-2,3 oxidosqualene into lanosterol, a crucial step in sterol nucleus formation. Beyond cholesterol biosynthesis, LSS may regulate lens protein aggregation, maintaining lens transparency. LSS's enzymatic action impacts fundamental and specialized cellular processes, underscoring its significance in diverse physiological contexts. Lanosterol synthase/LSS Protein, Human (P.pastoris, His) is the recombinant human-derived Lanosterol synthase/LSS protein, expressed by P. pastoris , with N-His labeled tag.
Background
Lanosterol synthase (LSS) emerges as a pivotal enzyme in the cholesterol biosynthesis pathway, orchestrating the cyclization of (S)-2,3 oxidosqualene into lanosterol. This catalytic activity holds a key role in the formation of the sterol nucleus, a critical step in cholesterol production. Beyond its role in cholesterol biosynthesis, LSS has been implicated in potential regulatory functions related to lens protein aggregation, suggesting a role in maintaining lens transparency. The enzymatic action of LSS not only contributes to fundamental cellular processes but may also extend its impact to specialized functions, highlighting its significance in cellular and physiological contexts.
Technical Parameters
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Species Human
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Source P. pastoris
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Tag N-His
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Accession
P48449 (T2-P732)
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Molecular Construction
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N-term
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His
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LSS (T2-P732)
Accession # P48449 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
LSS; 2,3-Epoxysqualene--Lanosterol Cyclase; Lanosterol Synthase; 2,3-Epoxysqualene-Lanosterol Cyclase; OSC; Oxidosqualene-Lanosterol Cyclase; Lanosterol Synthase (2,3-Oxidosqualene-Lanosterol Cyclase); CTRCT44; Oxidosqualene--Lanosterol Cyclase; HYPT14; H
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AA Sequence
TEGTCLRRRGGPYKTEPATDLGRWRLNCERGRQTWTYLQDERAGREQTGLEAYALGLDTKNYFKDLPKAHTAFEGALNGMTFYVGLQAEDGHWTGDYGGPLFLLPGLLITCHVARIPLPAGYREEIVRYLRSVQLPDGGWGLHIEDKSTVFGTALNYVSLRILGVGPDDPDLVRARNILHKKGGAVAIPSWGKFWLAVLNVYSWEGLNTLFPEMWLFPDWAPAHPSTLWCHCRQVYLPMSYCYAVRLSAAEDPLVQSLRQELYVEDFASIDWLAQRNNVAPDELYTPHSWLLRVVYALLNLYEHHHSAHLRQRAVQKLYEHIVADDRFTKSISIGPISKTINMLVRWYVDGPASTAFQEHVSRIPDYLWMGLDGMKMQGTNGSQIWDTAFAIQALLEAGGHHRPEFSSCLQKAHEFLRLSQVPDNPPDYQKYYRQMRKGGFSFSTLDCGWIVSDCTAEALKAVLLLQEKCPHVTEHIPRERLCDAVAVLLNMRNPDGGFATYETKRGGHLLELLNPSEVFGDIMIDYTYVECTSAVMQALKYFHKRFPEHRAAEIRETLTQGLEFCRRQQRADGSWEGSWGVCFTYGTWFGLEAFACMGQTYRDGTACAEVSRACDFLLSRQMADGGWGEDFESCEERRYLQSAQSQIHNTCWAMMGLMAVRHPDIEAQERGVRCLLEKQLPNGDWPQENIAGVFNKSCAISYTSYRNIFPIWALGRFSQLYPERALAGHP
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Molecular Weight
Approximately 86 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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