Copy Link Print Information

Lgr5/GPR49 Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

The Lgr5/GPR49 protein is a receptor for R-spondins that enhances the canonical Wnt signaling pathway and marks stem cells in the intestinal epithelium and hair follicles. Lgr5/GPR49 binds R-spondins (RSPO1-4), binds to phosphorylated LRP6 and Frizzled receptors, initiates Wnt signaling and amplifies target gene expression. Lgr5/GPR49 Protein, Human (HEK293, His) is the recombinant human-derived Lgr5/GPR49 protein, expressed by HEK293, with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Lgr5/GPR49 Protein, Human (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

The Lgr5/GPR49 protein is a receptor for R-spondins that enhances the canonical Wnt signaling pathway and marks stem cells in the intestinal epithelium and hair follicles. Lgr5/GPR49 binds R-spondins (RSPO1-4), binds to phosphorylated LRP6 and Frizzled receptors, initiates Wnt signaling and amplifies target gene expression. Lgr5/GPR49 Protein, Human (HEK293, His) is the recombinant human-derived Lgr5/GPR49 protein, expressed by HEK293, with C-His labeled tag.

Background

The Lgr5/GPR49 Protein serves as the receptor for R-spondins, functioning to potentiate the canonical Wnt signaling pathway while concurrently serving as a distinctive stem cell marker in the intestinal epithelium and hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3, or RSPO4), Lgr5/GPR49 associates with phosphorylated LRP6 and frizzled receptors activated by extracellular Wnt, initiating the canonical Wnt signaling pathway and amplifying the expression of target genes. In contrast to classical G-protein coupled receptors, Lgr5/GPR49 does not activate heterotrimeric G-proteins to transduce the signal, highlighting its unique regulatory role in Wnt signaling. It plays a crucial role in the development and maintenance of adult intestinal stem cells during postembryonic development and has been identified in a complex with RNF43 and RSPO1. Additionally, Lgr5/GPR49 interacts with other R-spondin ligands, including RSPO2, RSPO3, and RSPO4, emphasizing its diverse participation in cellular signaling pathways.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human R-Spondin 3 Protein, Fc Tag at 5 μg/mL (100 μL/well) can bind Human LGR5, His Tag with the EC50 of 15-35 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    O75473-1 (G22-R561)

  • Gene ID
    8549
  • Molecular Construction
    • N-term
    • Lgr5 (G22-R561)
      Accession # O75473-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    LGR5; G-Protein Coupled Receptor HG38; Prev. GPR49; G-Protein Coupled Receptor 49; Prev. GPR67; G-Protein Coupled Receptor 67; HG38; Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 5, Isoform CRA_b; FEX; Orphan G Protein-Coupled Receptor HG38; L

  • AA Sequence

    GSSPRSGVLLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTEIPVQAFRSLSALQAMTLALNKIHHIPDYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELGFHSNNIRSIPEKAFVGNPSLITIHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAFGVCENAYKISNQWNKGDNSSMDDLHKKDAGMFQAQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLIR

  • Predicted Molecular Mass

    62 kDa

  • Molecular Weight

    Approximately 65-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from 0.22 μm filtered solution of PBS, pH 7.4 with 10% trehalose as protectant.

Endotoxin Level

\

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • Lgr5/GPR49 Protein, Human (HEK293, His) Product Image
    1/16
  • Lgr5/GPR49 Protein, Human (HEK293, His) Product Image