LRRC15 Protein, Canine (HEK293, His)

LRRC15 protein actively modulates SARS-CoV-2 infectivity by directly interacting with the spike protein, inhibiting ACE2(+) cell infection. While not an entry receptor, LRRC15, expressed on nearby cells, sequesters virions, and its direct interaction with the spike protein's RBD domain leads to virion sequestration at the cell surface. LRRC15 Protein, Canine (HEK293, His) is the recombinant canine-derived LRRC15 protein, expressed by HEK293 , with C-His labeled tag.

The LRRC15 protein modulates the infectivity of SARS-CoV-2 by interacting with its spike protein. It does not function as an entry receptor for SARS-CoV-2, but instead, when expressed on nearby cells, it sequesters virions and inhibits SARS-CoV-2 infection of ACE2(+) cells in a trans manner. Furthermore, LRRC15 protein directly interacts with the RBD domain of the human coronavirus SARS-CoV-2 spike protein, leading to virion sequestration at the cell surface.

Canine

HEK293

C-8*His

XP_005639692.2 (Y47-G565)

488041  [NCBI]

Partial

YYGCPSECTCSRASQVECTGARIVAVPAPLPWNAMSLQILNTHITELNESPFLNISALIALRIEKNELSHIMPGAFRNLGSLRYLSLANNKLQVLPIGLFQGLNNLESLLLSSNQLVQIQPAHFSQFSNLKELQLHGNHLEYIPDGVFDHLVGLTKLNLGKNSLTHLSPRIFQRLGNLQVLRLYENRLSEIPMGTFDGCGNLQELALQQNQIGMLSPGLFHNNRNLQKLYLSNNHISQLPPGIFMQLPQLNRLTLFGNSLKELSPGIFGPMHNLRELWLYDNHITSLPDNVFSNLHQLQVLILSRNQINYISPDAFNGLVELRELSLHTNALQELDGSVFRMLVNLQNISLQNNRLRQLPGNIFANVNGLMTIQLQNNQLENLPMGIFDHLGNLCELRLYDNPWRCDSDILPLHNWLLLNKPRLGTDTLPVCFSPPSVRGQSLIIINVNVAVPSVQVPMIPEVPSYPETPQYPDTPSFPDTTSVSTTEFTSPVEDYTDLTTIEVTDDRSTWGMTQAQSG

70-80 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/sf9-cell.html  [Content Brief]