LRRC15 Protein, Human (Biotinylated, HEK293, His-Avi)
Based on 1 Customer Validation
LRRC15 protein actively modulates SARS-CoV-2 infectivity by directly interacting with the spike protein, inhibiting ACE2(+) cell infection. While not an entry receptor, LRRC15, expressed on nearby cells, sequesters virions, and its direct interaction with the spike protein's RBD domain leads to virion sequestration at the cell surface. LRRC15 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived LRRC15 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
LRRC15 protein actively modulates SARS-CoV-2 infectivity by directly interacting with the spike protein, inhibiting ACE2(+) cell infection. While not an entry receptor, LRRC15, expressed on nearby cells, sequesters virions, and its direct interaction with the spike protein's RBD domain leads to virion sequestration at the cell surface. LRRC15 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived LRRC15 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
Background
The LRRC15 protein modulates the infectivity of SARS-CoV-2 by interacting with its spike protein. It does not function as an entry receptor for SARS-CoV-2, but instead, when expressed on nearby cells, it sequesters virions and inhibits SARS-CoV-2 infection of ACE2(+) cells in a trans manner. Furthermore, LRRC15 protein directly interacts with the RBD domain of the human coronavirus SARS-CoV-2 spike protein, leading to virion sequestration at the cell surface.
Verified Bioactivity
1. Immobilized Anti-LRRC15 Antibody, hFc Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human LRRC15, His Tag with the EC50 of ≤68.5ng/mL determined by ELISA.
2. Loaded Samrotamab (HY-P99899) on AHC2 biosensor, can bind LRRC15 Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 5.759E-10 M as determined in BLI assay.
3. Immobilized Anti-LRRC15 Antibody, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human LRRC15, His Tag with the EC50 of 68.3ng/ml determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-8*His
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Accession
Q8TF66-1 (Y22-G538)
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Molecular Construction
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N-term
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LRRC15 (Y22-G538)
Accession # Q8TF66-1 -
8*His-Avi
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C-term
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Protein Length
Extracellular Domain
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Conjugation
Biotin
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Conjugate Method
The single lysine residue in Avitag is biotinylated through an enzymatic reaction.
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Synonyms
LRRC15; Leucine-Rich Repeat-Containing Protein 15; Leucine Rich Repeat Containing 15; HLib; LIB; Leucine-Rich Repeat Protein Induced By Beta Amyloid; Leucine-Rich Repeat Protein Induced By Beta-Amyloid Homolog
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AA Sequence
YHGCPSECTCSRASQVECTGARIVAVPTPLPWNAMSLQILNTHITELNESPFLNISALIALRIEKNELSRITPGAFRNLGSLRYLSLANNKLQVLPIGLFQGLDSLESLLLSSNQLLQIQPAHFSQCSNLKELQLHGNHLEYIPDGAFDHLVGLTKLNLGKNSLTHISPRVFQHLGNLQVLRLYENRLTDIPMGTFDGLVNLQELALQQNQIGLLSPGLFHNNHNLQRLYLSNNHISQLPPSVFMQLPQLNRLTLFGNSLKELSPGIFGPMPNLRELWLYDNHISSLPDNVFSNLRQLQVLILSRNQISFISPGAFNGLTELRELSLHTNALQDLDGNVFRMLANLQNISLQNNRLRQLPGNIFANVNGLMAIQLQNNQLENLPLGIFDHLGKLCELRLYDNPWRCDSDILPLRNWLLLNQPRLGTDTVPVCFSPANVRGQSLIIINVNVAVPSVHVPEVPSYPETPWYPDTPSYPDTTSVSSTTELTSPVEDYTDLTTIQVTDDRSVWGMTQAQSG
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Predicted Molecular Mass
60.7 kDa
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Molecular Weight
Approximately 70-95 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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