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  3. LRRC15 Protein, Human (Biotinylated, HEK293, His-Avi)

LRRC15 Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P78172
COA Handling Instructions

LRRC15 protein actively modulates SARS-CoV-2 infectivity by directly interacting with the spike protein, inhibiting ACE2(+) cell infection. While not an entry receptor, LRRC15, expressed on nearby cells, sequesters virions, and its direct interaction with the spike protein's RBD domain leads to virion sequestration at the cell surface. LRRC15 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived LRRC15 protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of LRRC15 Protein, Human (Biotinylated, HEK293, His-Avi) is 517 a.a., with molecular weight of 70-80 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

LRRC15 protein actively modulates SARS-CoV-2 infectivity by directly interacting with the spike protein, inhibiting ACE2(+) cell infection. While not an entry receptor, LRRC15, expressed on nearby cells, sequesters virions, and its direct interaction with the spike protein's RBD domain leads to virion sequestration at the cell surface. LRRC15 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived LRRC15 protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of LRRC15 Protein, Human (Biotinylated, HEK293, His-Avi) is 517 a.a., with molecular weight of 70-80 kDa.

Background

The LRRC15 protein modulates the infectivity of SARS-CoV-2 by interacting with its spike protein. It does not function as an entry receptor for SARS-CoV-2, but instead, when expressed on nearby cells, it sequesters virions and inhibits SARS-CoV-2 infection of ACE2(+) cells in a trans manner. Furthermore, LRRC15 protein directly interacts with the RBD domain of the human coronavirus SARS-CoV-2 spike protein, leading to virion sequestration at the cell surface.

Biological Activity

Immobilized Anti-LRRC15 Antibody, hFc Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human LRRC15, His Tag with the EC50 of ≤68.5ng/mL determined by ELISA.

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

Q8TF66 (Y22-G538)

Gene ID
Molecular Construction
N-term
LRRC15 (Y22-G538)
Accession # Q8TF66
His-Avi
C-term
Synonyms
LIB; LRRC15
AA Sequence

YHGCPSECTCSRASQVECTGARIVAVPTPLPWNAMSLQILNTHITELNESPFLNISALIALRIEKNELSRITPGAFRNLGSLRYLSLANNKLQVLPIGLFQGLDSLESLLLSSNQLLQIQPAHFSQCSNLKELQLHGNHLEYIPDGAFDHLVGLTKLNLGKNSLTHISPRVFQHLGNLQVLRLYENRLTDIPMGTFDGLVNLQELALQQNQIGLLSPGLFHNNHNLQRLYLSNNHISQLPPSVFMQLPQLNRLTLFGNSLKELSPGIFGPMPNLRELWLYDNHISSLPDNVFSNLRQLQVLILSRNQISFISPGAFNGLTELRELSLHTNALQDLDGNVFRMLANLQNISLQNNRLRQLPGNIFANVNGLMAIQLQNNQLENLPLGIFDHLGKLCELRLYDNPWRCDSDILPLRNWLLLNQPRLGTDTVPVCFSPANVRGQSLIIINVNVAVPSVHVPEVPSYPETPWYPDTPSYPDTTSVSSTTELTSPVEDYTDLTTIQVTDDRSVWGMTQAQSG

Molecular Weight

70-80 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

LRRC15 Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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LRRC15 Protein, Human (Biotinylated, HEK293, His-Avi)
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