MAN1B1 Protein, Human (HEK293, His)

MAN1B1, integral to glycoprotein quality control, trims a single alpha-1,2-linked mannose from Man(9)GlcNAc(2), producing Man(8)GlcNAc(2). In the ERQC, elevated enzyme concentrations allow further trimming to Man(5-6)GlcNAc(2). This enzymatic function is crucial for glycoprotein processing and quality control in the endoplasmic reticulum, ensuring proper folding and maturation. MAN1B1 Protein, Human (HEK293, His) is the recombinant human-derived MAN1B1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of MAN1B1 Protein, Human (HEK293, His) is 594 a.a., with molecular weight of 58-80 kDa.

The MAN1B1 protein plays a pivotal role in glycoprotein quality control, participating in the targeting of misfolded glycoproteins for degradation. Its primary enzymatic activity involves the trimming of a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2), resulting in the production of Man(8)GlcNAc(2). Notably, in the ER quality control compartment (ERQC) where enzyme concentrations are elevated, MAN1B1 can further trim the carbohydrates to Man(5-6)GlcNAc(2). This enzymatic function highlights its crucial involvement in the processing and quality control of glycoproteins within the endoplasmic reticulum, ensuring the proper folding and maturation of these proteins.

Human

HEK293

C-6*His

Q9UKM7 (D106-A699)

11253  [NCBI]

DHWKALAFRLEEEQKMRPEIAGLKPANPPVLPAPQKADTDPENLPEISSQKTQRHIQRGPPHLQIRPPSQDLKDGTQEEATKRQEAPVDPRPEGDPQRTVISWRGAVIEPEQGTELPSRRAEVPTKPPLPPARTQGTPVHLNYRQKGVIDVFLHAWKGYRKFAWGHDELKPVSRSFSEWFGLGLTLIDALDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIRILGGLLSAYHLSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNTEAHPLPIWTPA

58-80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 or 20 mM PB, 50 mM NaCl, 5% Sucrose, 5% Mannitol, 0.05% Tween 80, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.