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  4. MANSC1 Protein, Human (HEK293, His)

MANSC1 Protein, Human (HEK293, His) is the recombinant human-derived MANSC1 protein, expressed by HEK293, with C-6*His labeled tag.

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Beschreibung

MANSC1 Protein, Human (HEK293, His) is the recombinant human-derived MANSC1 protein, expressed by HEK293, with C-6*His labeled tag.
Background

MANSC (motif at N terminus with seven cysteines) is a novel domain with a well-conserved seven-cysteine motif that is present at the N terminus of membrane and extracellular proteins, including low-density lipoprotein receptor-related protein 11 (LRP-11), hepatocyte growth factor activator inhibitor 1 (HAI-1) and some uncharacterized proteins encoded by multicellular animals from Mollusca to Chordata[1].
Species

Human
Source

HEK293
Tag

C-6*His
Accession

AAH32998.1 (Q27-L385)
Gene ID
Molecular Construction
N-term
MANSC1 (Q27-L385)
Accession # AAH32998.1
6*His
C-term
Protein Length

Partial
Synonyms
rHuMANSC domain-containing protein 1/MANSC1, His ; MANSC Domain-Containing Protein 1; Loss of Heterozygosity 12 Chromosomal Region 3 Protein; MANSC1; LOH12CR3
AA Sequence

QNCLKKSLEDVVIDIQSSLSKGIRGNEPVYTSTQEDCINSCCSTKNISGDKACNLMIFDTRKTARQPNCYLFFCPNEEACPLKPAKGLMSYRIITDFPSLTRNLPSQELPQEDSLLHGQFSQAVTPLAHHHTDYSKPTDISWRDTLSQKFGSSDHLEKLFKMDEASAQLLAYKEKGHSQSSQFSSDQEIAHLLPENVSALPATVAVASPHTTSATPKPATLLPTNASVTPSGTSQPQLATTAPPVTTVTSQPPTTLISTVFTRAAATLQAMATTAVLTTTFQAPTDSKGSLETIPFTEISNLTLNTGNVYNPTALSMSNVESSTMNKTASWEGREASPGSSSQGSVPENQYGLPFEKWL

Predicted Molecular Mass
39.8 kDa
Molekulargewicht

Approximately 80-120 kDa,based on SDS-PAGE under reducing conditions.
Glycosylation
Yes
Reinheit

≥ 95%, as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Dokumentation
Handling Instructions (2659 KB)

MANSC1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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