MAOB Protein, Human (Active, sf9, His)
Based on 1 Customer Validation
MAOB Protein, Human (Active, sf9, His) is the recombinant human-derived MAOB protein, expressed by Sf9 insect cells, with N-His tag. This product contains detergent and is not suitable for cell-related experiments.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
MAOB Protein, Human (Active, sf9, His) is the recombinant human-derived MAOB protein, expressed by Sf9 insect cells, with N-His tag. This product contains detergent and is not suitable for cell-related experiments.
Background
MAOB is an enzyme that catalyzes the oxidative deamination of primary and some secondary amines (e.g., neurotransmitters) and participates in the metabolism of exogenous amines, including the tertiary amine neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), with concomitant reduction of oxygen to hydrogen peroxide. It plays a role in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. The enzyme preferentially degrades benzylamine and phenylethylamine. by similar: Studies further support its critical role in amine metabolism.
Verified Bioactivity
Measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine. The specific activity is >35 pmol/min/μg.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His
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Accession
P27338-1 (S2-V520)
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Gene ID4129
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Molecular Construction
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N-term
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His
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MAOB (S2-V520)
Accession # P27338-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
MAOB; Adrenalin Oxidase; Monoamine Oxidase B; Tyramine Oxidase; Amine Oxidase [Flavin-Containing] B; MAO, Platelet; Monoamine Oxidase Type B; Amine Oxidase; MAO-B; MAO, Brain
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AA Sequence
SNKCDVVVVGGGISGMAAAKLLHDSGLNVVVLEARDRVGGRTYTLRNQKVKYVDLGGSYVGPTQNRILRLAKELGLETYKVNEVERLIHHVKGKSYPFRGPFPPVWNPITYLDHNNFWRTMDDMGREIPSDAPWKAPLAEEWDNMTMKELLDKLCWTESAKQLATLFVNLCVTAETHEVSALWFLWYVKQCGGTTRIISTTNGGQERKFVGGSGQVSERIMDLLGDRVKLERPVIYIDQTRENVLVETLNHEMYEAKYVISAIPPTLGMKIHFNPPLPMMRNQMITRVPLGSVIKCIVYYKEPFWRKKDYCGTMIIDGEEAPVAYTLDDTKPEGNYAAIMGFILAHKARKLARLTKEERLKKLCELYAKVLGSLEALEPVHYEEKNWCEEQYSGGCYTTYFPPGILTQYGRVLRQPVDRIYFAGTETATHWSGYMEGAVEAGERAAREILHAMGKIPEDEIWQSEPESVDVPAQPITTTFLERHLPSVPGLLRLIGLTTIFSATALGFLAHKRGLLVRV
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Predicted Molecular Mass
60.6 kDa
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Molecular Weight
Approximately 55 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 0.05% DDM, 0.01% CHS, pH 7.5 with 10% glycerol as protectant. Due to the cytotoxicity of the detergent, this product is not appropriate for cell-based experiments.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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