MASP2 Protein, Cynomolgus (His)
Based on 1 Customer Validation
MASP2 Protein exhibits a deficiency in conserved residue(s) crucial for feature annotation propagation. MASP2 Protein, Cynomolgus (His) is the recombinant cynomolgus-derived MASP2 protein, expressed by E. coli , with C-His labeled tag.
- Species: Cynomolgus
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
MASP2 Protein exhibits a deficiency in conserved residue(s) crucial for feature annotation propagation. MASP2 Protein, Cynomolgus (His) is the recombinant cynomolgus-derived MASP2 protein, expressed by E. coli , with C-His labeled tag.
Background
The MASP2 protein is characterized by a deficiency in conserved residue(s) essential for the propagation of feature annotation.
Verified Bioactivity
Immobilized Cynomolgus MASP2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 11.9 ng/ml determined by ELISA.
Technical Parameters
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Species Cynomolgus
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Source E. coli
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Tag C-6*His
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Accession
A0A2K5UJY0 (T287-F686)
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Molecular Construction
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N-term
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MASP2 (T287-F686)
Accession # A0A2K5UJY0 -
6*His
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C-term
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Protein Length
Partial
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Synonyms
MASP2; Mannan-Binding Lectin Serine Protease 1 Pseudogene 1; Prev. MASP1P1; Mannose-Binding Lectin Associated Protein 19; Mannan-Binding Lectin Serine Protease 2; MBL-Associated Serine Protease 2; Mannose-Binding Protein-Associated Serine Protease 2; MASP
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AA Sequence
TGWKIHYTSTAQPCPYPMAPPNGHLSPVQAKYILKDSFSIFCEPGYELLQGHLPLKSFAAVCQKDGSWDQPMPSCSIVDCGPPDDLPSGRVEYITGPEVTTYKAVIQYSCEETFYTMKVNDGKYVCEADGFWTSSKGERSPPVCEPVCGLSARTTGGRIYGGQKAKPGDFPWQVLILGGSTAAGALLYDNWVLTAAHAIYEQKHDASSLDIRLGALKRLSPHYTQAWAEAVFIHEGYTHDAGFDNDIALIKLNNKVVINSNITPICLPRKEAESFMRTDDIGTASGWGLTQRGLLARNLMYVDIPIVDHQKCTAAYEKPPYSGGSVTANMLCAGLESGGKDSCRGDSGGALVFLDNETQRWFVGGIVSWGSMNCGEAGQYGVYTKVINYIPWIKNIISNF
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Predicted Molecular Mass
46.8 kDa
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Molecular Weight
Approximately 21-24 kDa & 28-30 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 200 mM NaCl, pH 9.0. Normally 8% trehalose/8% mannitol is added as protectant before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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