MC4R Protein, Human (Cell-Free, His)
MC4R is a receptor for adrenocorticotropic hormone and α, β, and γ-MSH and plays a key role in regulating energy homeostasis and somatic cell growth. Mediated by G proteins, it forms disulfide-linked homodimers and higher-order oligomers. MC4R Protein, Human (Cell-Free, His) is the recombinant human-derived MC4R protein, expressed by E. coli Cell-free , with C-10*His labeled tag.
- Species: Human
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
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Biological Activity
Description
MC4R is a receptor for adrenocorticotropic hormone and α, β, and γ-MSH and plays a key role in regulating energy homeostasis and somatic cell growth. Mediated by G proteins, it forms disulfide-linked homodimers and higher-order oligomers. MC4R Protein, Human (Cell-Free, His) is the recombinant human-derived MC4R protein, expressed by E. coli Cell-free , with C-10*His labeled tag.
Background
MC4R, a receptor specific to the heptapeptide core shared by adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH, plays a pivotal role in regulating energy homeostasis and somatic growth. Mediated by G proteins that stimulate adenylate cyclase to generate cAMP, this receptor forms homodimers that are disulfide-linked and can further assemble into higher-order oligomers. MC4R interacts with ATRNL1 and engages in an interaction with MGRN1, inhibiting agonist-induced cAMP production by competing with GNAS-binding. Additionally, it forms complexes with MRAP and MRAP2, enhancing ligand sensitivity and cAMP generation, thereby contributing to its multifaceted regulatory functions.
Technical Parameters
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Species Human
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Source E. coli Cell-free
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Tag C-10*His
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Accession
P32245 (M1-Y332)
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Gene ID17202
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Molecular Construction
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N-term
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MC4R (M1-Y332)
Accession # P32245 -
10*His
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C-term
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Protein Length
Full Length
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Synonyms
MC4R; Melanocortin receptor 4; MC4-R; BMIQ20; Mutant Melanocortin-4 Receptor; Melanocortin 4 Receptor; Mutated Melanocortin Receptor 4
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AA Sequence
MVNSTHRGMHTSLHLWNRSSYRLHSNASESLGKGYSDGGCYEQLFVSPEVFVTLGVISLLENILVIVAIAKNKNLHSPMYFFICSLAVADMLVSVSNGSETIVITLLNSTDTDAQSFTVNIDNVIDSVICSSLLASICSLLSIAVDRYFTIFYALQYHNIMTVKRVGIIISCIWAACTVSGILFIIYSDSSAVIICLITMFFTMLALMASLYVHMFLMARLHIKRIAVLPGTGAIRQGANMKGAITLTILIGVFVVCWAPFFLHLIFYISCPQNPYCVCFMSHFNLYLILIMCNSIIDPLIYALRSQELRKTFKEIICCYPLGGLCDLSSRY
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Predicted Molecular Mass
38.3 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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