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MCD Protein, Human (E58A, K59A, E278A, E279A, K280A, His)

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MCD proteins catalyze the conversion of malonyl-CoA to acetyl-CoA, ensuring that only methylmalonyl-CoA serves as a substrate for fatty acid synthase, producing fatty acids with multiple methyl side chains. MCD Protein, Human (E58A, K59A, E278A, E279A, K280A, His) is the recombinant human-derived MCD protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • MCD Protein, Human (E58A, K59A, E278A, E279A, K280A, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

MCD proteins catalyze the conversion of malonyl-CoA to acetyl-CoA, ensuring that only methylmalonyl-CoA serves as a substrate for fatty acid synthase, producing fatty acids with multiple methyl side chains. MCD Protein, Human (E58A, K59A, E278A, E279A, K280A, His) is the recombinant human-derived MCD protein, expressed by E. coli , with N-6*His labeled tag.

Background

The MCD protein plays a pivotal role in cellular metabolism by catalyzing the conversion of malonyl-CoA to acetyl-CoA. Particularly significant in fatty acid biosynthesis, MCD selectively eliminates malonyl-CoA, ensuring that methyl-malonyl-CoA becomes the exclusive substrate for fatty acid synthase. This selective removal mechanism contributes to the production of fatty acids with multiple methyl side chains. In peroxisomes, MCD is implicated in the degradation of intraperoxisomal malonyl-CoA, a byproduct of peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids. Beyond its involvement in lipid metabolism, MCD influences the metabolic balance between glucose and lipid oxidation in muscle, independent of insulin signaling alterations. Additionally, MCD may play a role in mitigating ischemic injury by promoting glucose oxidation, highlighting its multifaceted impact on cellular functions and metabolic pathways.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    O95822 (M40-S491, E58A, K59A, E278A, E279A, K280A)

  • Gene ID
    23417
  • Molecular Construction
    • N-term
    • 6*His
    • MCD (M40-S491)
      Accession # O95822
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    MLYCD; Malonyl-CoA Decarboxylase, Mitochondrial; Malonyl-CoA Decarboxylase; HMCD; MCD; Malonyl Coenzyme A Decarboxylase

  • AA Sequence

    MDELLRRAVPPTPAYELRAATPAPAEGQCADFVSFYGGLAETAQRAELLGRLARGFGVDHGQVAEQSAGVLHLRQQQREAAVLLQAEDRLRYALVPRYRGLFHHISKLDGGVRFLVQLRADLLEAQALKLVEGPDVREMNGVLKGMLSEWFSSGFLNLERVTWHSPCEVLQKISEAEAVHPVKNWMDMKRRVGPYRRCYFFSHCSTPGEPLVVLHVALTGDISSNIQAIVKEHPPSETAAANKITAAIFYSISLTQQGLQGVELGTFLIKRVVKELQREFPHLGVFSSLSPIPGFTKWLLGLLNSQTKEHGRNELFTDSECKEISEITGGPINETLKLLLSSSEWVQSEKLVRALQTPLMRLCAWYLYGEKHRGYALNPVANFHLQNGAVLWRINWMADVSLRGITGSCGLMANYRYFLEETGPNSTSYLGSKIIKASEQVLSLVAQFQKNS

  • Predicted Molecular Mass

    52.4 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • MCD Protein, Human (E58A, K59A, E278A, E279A, K280A, His) Product Image
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  • MCD Protein, Human (E58A, K59A, E278A, E279A, K280A, His) Product Image