Meprin alpha/MEP1A Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
Meprin alpha (MEP1A) protein functions as a metalloendopeptidase in the membrane. It is found in structures like the gut and metanephros. MEP1A primarily expresses in the adult kidney and large intestine, indicating potential tissue-specific functions. Meprin alpha/MEP1A Protein, Mouse (HEK293, His) is the recombinant mouse-derived Meprin alpha/MEP1A protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Meprin alpha (MEP1A) protein functions as a metalloendopeptidase in the membrane. It is found in structures like the gut and metanephros. MEP1A primarily expresses in the adult kidney and large intestine, indicating potential tissue-specific functions. Meprin alpha/MEP1A Protein, Mouse (HEK293, His) is the recombinant mouse-derived Meprin alpha/MEP1A protein, expressed by HEK293 , with C-His labeled tag.
Background
Meprin alpha (MEP1A) protein is predicted to exhibit metalloendopeptidase activity and is primarily located in the membrane. It is expressed in various structures, including the gut and metanephros. The expression of MEP1A is notably biased, with higher expression observed in the adult kidney and large intestine, suggesting potential roles in these tissues.
Verified Bioactivity
Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH. The specific activity is 324.93 pmol/min/μg.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-His
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Accession
NP_032611.2 (V34-R615)
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Molecular Construction
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N-term
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MEP1A (V34-R615)
Accession # NP_032611.2 -
His
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
MEP1A; Meprin A, Alpha (PABA Peptide Hydrolase); Meprin A Subunit Alpha; PPH Alpha; PABA Peptide Hydrolase; BA268F1.1 (Meprin A Alpha (PABA Peptide Hydrolase)); Endopeptidase-2; Metalloendopeptidase; PPHA; Meprin A Subunit; N-Benzoyl-L-Tyrosyl-P-Amino-Ben
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AA Sequence
VSIKHLLNGSDHDTDVGEQKDIFEINLAAGLNLFQGDILLPRTRNAMRDPSSRWKLPIPYILADNLELNAKGAILHAFEMFRLKSCVDFKPYEGESSYIIFQKLSGCWSMIGDQQVGQNISIGEGCDFKATIEHEILHALGFFHEQSRTDRDDYVNIWWDQIITDYEHNFNTYDDNTITDLNTPYDYESLMHYGPFSFNKNESIPTITTKIPEFNTIIGQLPDFSAIDLIRLNRMYNCTATHTLLDHCDFEKTNVCGMIQGTRDDADWAHGDSSQPEQVDHTLVGQCKGAGYFMFFNTSLGARGEAALLESRILYPKRKQQCLQFFYKMTGSPADRFEVWVRRDDNAGKVRQLAKIQTFQGDSDHNWKIAHVTLNEEKKFRYVFLGTKGDPGNSSGGIYLDDITLTETPCPAGVWTIRNISQILENTVKGDKLVSPRFYNSEGYGVGVTLYPNGRITSNSGFLGLTFHLYSGDNDAILEWPVENRQAIMTILDQEADTRNRMSLTLMFTTSKNQTSSAINGSVIWDRPSKVGVYDKDCDCFRSLDWGWGQAISHQLLKRRNFLKGDSLIIFVDFKDLTHLNR
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Molecular Weight
Approximately 80-90 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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