MME Protein, Human (HEK293, N-His)
Based on 1 Customer Validation
MME proteins exhibit thermolysin-like specificity and primarily degrade polypeptides of up to 30 amino acids. Crucially, it plays a key role in cleaving the Gly-Phe bond to degrade opioid peptides, including Met-enkephalin and Leu-enkephalin. MME Protein, Human (HEK293, N-His) is the recombinant human-derived MME protein, expressed by HEK293 , with N-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
MME proteins exhibit thermolysin-like specificity and primarily degrade polypeptides of up to 30 amino acids. Crucially, it plays a key role in cleaving the Gly-Phe bond to degrade opioid peptides, including Met-enkephalin and Leu-enkephalin. MME Protein, Human (HEK293, N-His) is the recombinant human-derived MME protein, expressed by HEK293 , with N-His labeled tag.
Background
The MME Protein demonstrates a thermolysin-like specificity, predominantly acting on polypeptides of up to 30 amino acids. Biologically crucial, it plays a key role in the degradation of opioid peptides, including Met- and Leu-enkephalins, achieved through the cleavage of a Gly-Phe bond. Additionally, MME catalyzes the cleavage of bradykinin, substance P, and neurotensin peptides and is capable of cleaving angiotensin-1, angiotensin-2, and angiotensin 1-9. The protein is involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). Furthermore, MME displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Verified Bioactivity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp) -OH. The specific activity is ≥1500 pmol/min/μg.
Technical Parameters
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Species Human
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Source HEK293
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Tag N-His
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Accession
P08473 (Y52-W750)
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Gene ID4311
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Molecular Construction
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N-term
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His
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MME (Y52-W750)
Accession # P08473 -
C-term
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Protein Length
Extracellular Domain
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Synonyms
MME; Neprilysin-390; Membrane Metalloendopeptidase; Neprilysin-411; Neprilysin; SFE; CALLA; Membrane Metallo-Endopeptidase (Neutral Endopeptidase, Enkephalinase, CALLA, CD10); NEP; Membrane Metallo-Endopeptidase Variant 2; Neutral Endopeptidase 24.11; Alternative Protein MME; Skin Fibroblast Elastase; CD10 Antigen; Atriopeptidase; MME Protein; Enkephalinase; CMT2T; CD10; SCA43; Common Acute Lymphocytic Leukemia Antigen; EPN; Neutral Endopeptidase
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AA Sequence
YDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW
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Predicted Molecular Mass
80.9 kDa
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Molecular Weight
Approximately 81-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50 mM MES, 100 mM NaCl, 1 mM ZnCl2, 10% glycerol, pH 6.5.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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