MMP-14 Protein, Human (C-His)
Based on 1 Customer Validation
MMP-14 protein is an endopeptidase that degrades collagen and activates progelatinase A.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
MMP-14 protein is an endopeptidase that degrades collagen and activates progelatinase A.
Background
MMP-14 Protein serves as a critical endopeptidase, targeting various components of the extracellular matrix, notably collagen, and playing a pivotal role in pericellular collagenolysis during development, contributing to the modeling of both skeletal and extraskeletal connective tissues. Additionally, MMP-14 activates progelatinase A, acting as a key player in the intricate regulation of the extracellular matrix. Beyond its matrix-degrading functions, MMP-14 is implicated in actin cytoskeleton reorganization by cleaving PTK7, highlighting its involvement in cellular processes beyond matrix remodeling. Furthermore, MMP-14 acts as a positive regulator of cell growth and migration through the activation of MMP15 and plays a crucial role in the formation of fibrovascular tissues in conjunction with pro-MMP2. Notably, MMP-14 cleaves ADGRB1, releasing vasculostatin-40, which exerts anti-angiogenic effects by inhibiting angiogenesis. This multifaceted functionality underscores MMP-14's significance in orchestrating diverse cellular processes and its potential as a therapeutic target in various physiological contexts.
Verified Bioactivity
Measured by its ability to cleave the fluorogenic peptide substrate Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala- Arg-NH2 (HY-P4931) that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is >450 pmol/min/µg. (Activation description: The proenzyme needs to be activated by Furin (HY-P78747) for an activated form)
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source E. coli
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Tag C-10*His
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Accession
P50281 (A21-G284)
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Gene ID4323
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Molecular Construction
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N-term
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MMP-14 (A21-G284)
Accession # P50281 -
10*His
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C-term
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Protein Length
Partial
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Synonyms
MMP14; MMP-X1; Matrix Metallopeptidase 14; MT1MMP; MT1-MMP; MTMMP1; Matrix Metalloproteinase-14; Matrix Metalloproteinase 14 (Membrane-Inserted); Matrix Metallopeptidase 14 (Membrane-Inserted); Membrane Type 1-Matrix Metalloproteinase; Membrane-Type-1 Mat
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Predicted Molecular Mass
31 kDa
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Molecular Weight
Approximately 31 kDa, based on SDS-PAGE under reducing conditions.
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Structure/Form
Pro and catalytic domains
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, pH 8.0, 10% glycerol, 2 mM CaCl2.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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