MRGPRX2 Protein, Human (Cell-Free, His)

The MRGPRX2 protein functions as a mast cell-specific receptor that responds to basic secretagogues and peptides with a cyclic tetrahydroisoquinoline (THIQ) structure. It recognizes ligands such as nonsteroidal neuromuscular blocking drugs (NMBD) and various peptides and alkaloids, including cortistatin-14, proadrenomedullin N-telopeptide PAMP-12, and the antimicrobial protein LL-37. MRGPRX2 Protein, Human (Cell-Free, His) is the recombinant human-derived MRGPRX2 protein, expressed by E. coli Cell-free , with N-6*His labeled tag. The total length of MRGPRX2 Protein, Human (Cell-Free, His) is 330 a.a., with molecular weight of 43.1 kDa.

MRGPRX2 Protein, a mast cell-specific receptor, functions as a receptor for basic secretagogues, including cationic amphiphilic drugs and peptides with a basic head group and a hydrophobic core. It recognizes small molecules with a cyclized tetrahydroisoquinoline, such as non-steroidal neuromuscular blocking drugs like tubocurarine and atracurium. Upon binding, MRGPRX2 mediates pseudo-allergic reactions marked by histamine release, inflammation, and airway contraction. Additionally, it serves as a receptor for various ligands, including peptides (cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and PAMP-20, antibacterial protein LL-37, PMX-53 peptide, and beta-defensins) and alkaloids like complanadine A.

Human

E. coli Cell-free

N-6*His

Q96LB1 (M1-V330)

117194

MDPTTPAWGTESTTVNGNDQALLLLCGKETLIPVFLILFIALVGLVGNGFVLWLLGFRMRRNAFSVYVLSLAGADFLFLCFQIINCLVYLSNFFCSISINFPSFFTTVMTCAYLAGLSMLSTVSTERCLSVLWPIWYRCRRPRHLSAVVCVLLWALSLLLSILEGKFCGFLFSDGDSGWCQTFDFITAAWLIFLFMVLCGSSLALLVRILCGSRGLPLTRLYLTILLTVLVFLLCGLPFGIQWFLILWIWKDSDVLFCHIHPVSVVLSSLNSSANPIIYFFVGSFRKQWRLQQPILKLALQRALQDIAEVDHSEGCFRQGTPEMSRSSLV

43.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.