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MRGPRX2 Protein, Human (Cell-Free, His)

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The MRGPRX2 protein functions as a mast cell-specific receptor that responds to basic secretagogues and peptides with a cyclic tetrahydroisoquinoline (THIQ) structure. It recognizes ligands such as nonsteroidal neuromuscular blocking drugs (NMBD) and various peptides and alkaloids, including cortistatin-14, proadrenomedullin N-telopeptide PAMP-12, and the antimicrobial protein LL-37. MRGPRX2 Protein, Human (Cell-Free, His) is the recombinant human-derived MRGPRX2 protein, expressed by E. coli Cell-free , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli Cell-free
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The MRGPRX2 protein functions as a mast cell-specific receptor that responds to basic secretagogues and peptides with a cyclic tetrahydroisoquinoline (THIQ) structure. It recognizes ligands such as nonsteroidal neuromuscular blocking drugs (NMBD) and various peptides and alkaloids, including cortistatin-14, proadrenomedullin N-telopeptide PAMP-12, and the antimicrobial protein LL-37. MRGPRX2 Protein, Human (Cell-Free, His) is the recombinant human-derived MRGPRX2 protein, expressed by E. coli Cell-free , with N-6*His labeled tag.

Background

MRGPRX2 Protein, a mast cell-specific receptor, functions as a receptor for basic secretagogues, including cationic amphiphilic drugs and peptides with a basic head group and a hydrophobic core. It recognizes small molecules with a cyclized tetrahydroisoquinoline, such as non-steroidal neuromuscular blocking drugs like tubocurarine and atracurium. Upon binding, MRGPRX2 mediates pseudo-allergic reactions marked by histamine release, inflammation, and airway contraction. Additionally, it serves as a receptor for various ligands, including peptides (cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and PAMP-20, antibacterial protein LL-37, PMX-53 peptide, and beta-defensins) and alkaloids like complanadine A.

Technical Parameters

  • Species Human
  • Source E. coli Cell-free
  • Tag N-6*His
  • Accession

    Q96LB1 (M1-V330)

  • Gene ID
    117194
  • Molecular Construction
    • N-term
    • 6*His
    • MRGPRX2 (M1-V330)
      Accession # Q96LB1
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    MRGPRX2; MAS-Related GPR, Member X2; MAS Related GPR Family Member X2; Mas-Related G Protein-Coupled Receptor G3; MRGX2; G Protein-Coupled Receptor MRGX2; Mas-Related G-Protein Coupled Receptor Member X2; MGRG3

  • AA Sequence

    MDPTTPAWGTESTTVNGNDQALLLLCGKETLIPVFLILFIALVGLVGNGFVLWLLGFRMRRNAFSVYVLSLAGADFLFLCFQIINCLVYLSNFFCSISINFPSFFTTVMTCAYLAGLSMLSTVSTERCLSVLWPIWYRCRRPRHLSAVVCVLLWALSLLLSILEGKFCGFLFSDGDSGWCQTFDFITAAWLIFLFMVLCGSSLALLVRILCGSRGLPLTRLYLTILLTVLVFLLCGLPFGIQWFLILWIWKDSDVLFCHIHPVSVVLSSLNSSANPIIYFFVGSFRKQWRLQQPILKLALQRALQDIAEVDHSEGCFRQGTPEMSRSSLV

  • Predicted Molecular Mass

    43.1 kDa

  • Molecular Weight

    Approximately 44 kDa (Monomer) & 116 kDa (Dimer), based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij-78, 6% trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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