MRGPRX2 Protein, Human (Cell-Free, His)

The MRGPRX2 protein functions as a mast cell-specific receptor that responds to basic secretagogues and peptides with a cyclic tetrahydroisoquinoline (THIQ) structure. It recognizes ligands such as nonsteroidal neuromuscular blocking drugs (NMBD) and various peptides and alkaloids, including cortistatin-14, proadrenomedullin N-telopeptide PAMP-12, and the antimicrobial protein LL-37. MRGPRX2 Protein, Human (Cell-Free, His) is the recombinant human-derived MRGPRX2 protein, expressed by E. coli Cell-free , with N-6*His labeled tag.

MRGPRX2 Protein, a mast cell-specific receptor, functions as a receptor for basic secretagogues, including cationic amphiphilic drugs and peptides with a basic head group and a hydrophobic core. It recognizes small molecules with a cyclized tetrahydroisoquinoline, such as non-steroidal neuromuscular blocking drugs like tubocurarine and atracurium. Upon binding, MRGPRX2 mediates pseudo-allergic reactions marked by histamine release, inflammation, and airway contraction. Additionally, it serves as a receptor for various ligands, including peptides (cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and PAMP-20, antibacterial protein LL-37, PMX-53 peptide, and beta-defensins) and alkaloids like complanadine A.

Human

E. coli Cell-free

N-6*His

Q96LB1 (M1-V330)

117194

Full Length

MDPTTPAWGTESTTVNGNDQALLLLCGKETLIPVFLILFIALVGLVGNGFVLWLLGFRMRRNAFSVYVLSLAGADFLFLCFQIINCLVYLSNFFCSISINFPSFFTTVMTCAYLAGLSMLSTVSTERCLSVLWPIWYRCRRPRHLSAVVCVLLWALSLLLSILEGKFCGFLFSDGDSGWCQTFDFITAAWLIFLFMVLCGSSLALLVRILCGSRGLPLTRLYLTILLTVLVFLLCGLPFGIQWFLILWIWKDSDVLFCHIHPVSVVLSSLNSSANPIIYFFVGSFRKQWRLQQPILKLALQRALQDIAEVDHSEGCFRQGTPEMSRSSLV

Monomer:44 kDa Dimer: 116 kDa It is speculated that the protein forms a dimeric structure.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij-78, 6% trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.