MYCN Protein, Human (sf9, His)

The MYCN protein is critical in neuroblastoma cells and can positively regulate MYCNOS transcription through DNA binding, requiring heterodimerization with MAX. Interactions with KDM5A, KDM5B, and HUWE1 indicate involvement in multiple cellular processes. MYCN Protein, Human (Sf9, His) is the recombinant human-derived MYCN, expressed by Sf9 insect cells, with C-6*His labeled tag.

MYCN, a critical player in neuroblastoma cells, exerts positive regulatory control over the transcription of MYCNOS. This regulatory function is contingent on efficient DNA binding, necessitating dimerization with another basic helix-loop-helix (bHLH) protein. Notably, MYCN forms a heterodimer with MAX for effective DNA binding. The intricate network of interactions involves KDM5A, KDM5B, and HUWE1, indicating its involvement in various cellular processes. Furthermore, MYCN engages in phospho-independent interactions with AURKA, triggering AURKA activation, while also interacting with MYCNOS. The competitive dynamics between AURKA and FBXW7 for binding to unphosphorylated MYCN unveil additional layers of regulatory complexity, emphasizing the multifaceted nature of MYCN's involvement in cellular pathways.

Human

Sf9 insect cells

C-6*His

P04198 (M1-C464)

4613

Full Length

MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC

65 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.