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MYCN Protein, Human (sf9, His)

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The MYCN protein is critical in neuroblastoma cells and can positively regulate MYCNOS transcription through DNA binding, requiring heterodimerization with MAX. Interactions with KDM5A, KDM5B, and HUWE1 indicate involvement in multiple cellular processes. MYCN Protein, Human (Sf9, His) is the recombinant human-derived MYCN, expressed by Sf9 insect cells, with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • MYCN Protein, Human (sf9, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

The MYCN protein is critical in neuroblastoma cells and can positively regulate MYCNOS transcription through DNA binding, requiring heterodimerization with MAX. Interactions with KDM5A, KDM5B, and HUWE1 indicate involvement in multiple cellular processes. MYCN Protein, Human (Sf9, His) is the recombinant human-derived MYCN, expressed by Sf9 insect cells, with C-6*His labeled tag.

Background

MYCN, a critical player in neuroblastoma cells, exerts positive regulatory control over the transcription of MYCNOS. This regulatory function is contingent on efficient DNA binding, necessitating dimerization with another basic helix-loop-helix (bHLH) protein. Notably, MYCN forms a heterodimer with MAX for effective DNA binding. The intricate network of interactions involves KDM5A, KDM5B, and HUWE1, indicating its involvement in various cellular processes. Furthermore, MYCN engages in phospho-independent interactions with AURKA, triggering AURKA activation, while also interacting with MYCNOS. The competitive dynamics between AURKA and FBXW7 for binding to unphosphorylated MYCN unveil additional layers of regulatory complexity, emphasizing the multifaceted nature of MYCN's involvement in cellular pathways.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-6*His
  • Accession

    P04198 (M1-C464)

  • Gene ID
    4613
  • Molecular Construction
    • N-term
    • MYCN (M1-C464)
      Accession # P04198
    • 6*His
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    MYCN; Neuroblastoma MYC Oncogene; Prev. NMYC; Oncogene NMYC; BHLHe37; MYCNsORF; N-Myc; MYCNsPEP; V-Myc Avian Myelocytomatosis Viral Oncogene Neuroblastoma Derived Homolog; BHLHE37; Class E Basic Helix-Loop-Helix Protein 37; FGLDS1; N-Myc Proto-Oncogene Pr

  • AA Sequence

    MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC

  • Predicted Molecular Mass

    52.4 kDa

  • Molecular Weight

    Approximately 65 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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