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Netrin receptor DCC/DCC Protein, Human (HEK293, His)

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The netrin receptor DCC guides axonal attraction in neuronal growth cones during nervous system development. Interaction with UNC5 protein induces axonal repulsion. Netrin receptor DCC/DCC Protein, Human (HEK293, His) is the recombinant human-derived Netrin receptor DCC/DCC protein, expressed by HEK293 , with C-10*His labeled tag.

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  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Netrin receptor DCC/DCC Protein, Human (HEK293, His)
  • Experimental Validation Results for Netrin receptor DCC/DCC Protein, Human (HEK293, His)
  • Netrin receptor DCC/DCC Protein, Human (HEK293, His) Product Image
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609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The netrin receptor DCC guides axonal attraction in neuronal growth cones during nervous system development. Interaction with UNC5 protein induces axonal repulsion. Netrin receptor DCC/DCC Protein, Human (HEK293, His) is the recombinant human-derived Netrin receptor DCC/DCC protein, expressed by HEK293 , with C-10*His labeled tag.

Background

DCC, a critical receptor for netrin, plays a pivotal role in axon guidance by mediating axon attraction in neuronal growth cones during nervous system development upon ligand binding. Its intricate associations with UNC5 proteins may trigger signaling pathways leading to axon repulsion. Additionally, DCC acts as a dependence receptor, instigating apoptosis when not bound to the netrin ligand. Beyond its role in neural development, DCC is implicated as a tumor suppressor gene. The receptor engages in diverse protein interactions, including the cytoplasmic parts of UNC5A, UNC5B, UNC5C, and possibly UNC5D, as well as with DSCAM, PTK2/FAK1, MAPK1, NTN1, MYO10, CBLN4, SIAH1, and SIAH2. These interactions underscore the multifaceted functions of DCC in orchestrating axon guidance, apoptosis, and potential tumor suppression.

Verified Bioactivity

Immobilized Recombinant Human DCC at 1 μg/ml (100 μl/well) can bind Biotinylated Recombinant Human Netrin-1 Protein. The ED50 for this effect is 85.29 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Recombinant Human DCC at 1 μg/mL (100 μl/well) can bind Biotinylated Recombinant Human Netrin-1 Protein. The ED50 for this effect is 85.29 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    P43146 (F32-N1097)

  • Gene ID
    1630  [NCBI]
  • Molecular Construction
    • N-term
    • DCC (F32-N1097)
      Accession # P43146
    • 10*His
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    DCC; Immunoglobulin Superfamily, DCC Subclass, Member 1; DCC Netrin 1 Receptor; Putative Colorectal Tumor Suppressor; IGDCC1; Deleted In Colorectal Cancer Protein; Tumor Suppressor Protein DCC; DCC Protein; Netrin Receptor DCC; HGPPS2; NTN1R1; CRC18; Immu

  • AA Sequence

    FQIKAFTALRFLSEPSDAVTMRGGNVLLDCSAESDRGVPVIKWKKDGIHLALGMDERKQQLSNGSLLIQNILHSRHHKPDEGLYQCEASLGDSGSIISRTAKVAVAGPLRFLSQTESVTAFMGDTVLLKCEVIGEPMPTIHWQKNQQDLTPIPGDSRVVVLPSGALQISRLQPGDIGIYRCSARNPASSRTGNEAEVRILSDPGLHRQLYFLQRPSNVVAIEGKDAVLECCVSGYPPPSFTWLRGEEVIQLRSKKYSLLGGSNLLISNVTDDDSGMYTCVVTYKNENISASAELTVLVPPWFLNHPSNLYAYESMDIEFECTVSGKPVPTVNWMKNGDVVIPSDYFQIVGGSNLRILGVVKSDEGFYQCVAENEAGNAQTSAQLIVPKPAIPSSSVLPSAPRDVVPVLVSSRFVRLSWRPPAEAKGNIQTFTVFFSREGDNRERALNTTQPGSLQLTVGNLKPEAMYTFRVVAYNEWGPGESSQPIKVATQPELQVPGPVENLQAVSTSPTSILITWEPPAYANGPVQGYRLFCTEVSTGKEQNIEVDGLSYKLEGLKKFTEYSLRFLAYNRYGPGVSTDDITVVTLSDVPSAPPQNVSLEVVNSRSIKVSWLPPPSGTQNGFITGYKIRHRKTTRRGEMETLEPNNLWYLFTGLEKGSQYSFQVSAMTVNGTGPPSNWYTAETPENDLDESQVPDQPSSLHVRPQTNCIIMSWTPPLNPNIVVRGYIIGYGVGSPYAETVRVDSKQRYYSIERLESSSHYVISLKAFNNAGEGVPLYESATTRSITDPTDPVDYYPLLDDFPTSVPDLSTPMLPPVGVQAVALTHDAVRVSWADNSVPKNQKTSEVRLYTVRWRTSFSASAKYKSEDTTSLSYTATGLKPNTMYEFSVMVTKNRRSSTWSMTAHATTYEAAPTSAPKDLTVITREGKPRAVIVSWQPPLEANGKITAYILFYTLDKNIPIDDWIMETISGDRLTHQIMDLNLDTMYYFRIQARNSKGVGPLSDPILFRTLKVEHPDKMANDQGRHGDGGYWPVDTNLIDRSTLNEPPIGQMHPPHGSVTPQKNSN

  • Molecular Weight

    Approximately 125-150 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Recombinant Human DCC at 1 μg/mL (100 μl/well) can bind Biotinylated Recombinant Human Netrin-1 Protein. The ED50 for this effect is 85.29 ng/mL.

  • Netrin receptor DCC/DCC Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Netrin receptor DCC/DCC Protein, Human (HEK293, His)
  • Experimental Validation Results for Netrin receptor DCC/DCC Protein, Human (HEK293, His)
  • Netrin receptor DCC/DCC Protein, Human (HEK293, His) Product Image