Netrin receptor DCC/DCC Protein, Human (HEK293, His)

The Netrin receptor DCC guides axon attraction in neuronal growth cones during nervous system development. Interactions with UNC5 proteins induce axon repulsion. Acting as a dependence receptor, unbound DCC triggers apoptosis. Beyond neural development, DCC serves as a tumor suppressor gene, engaging in diverse protein interactions. These interactions underscore DCC's multifaceted role in orchestrating axon guidance, apoptosis, and potential tumor suppression. Netrin receptor DCC/DCC Protein, Human (HEK293, His) is the recombinant human-derived Netrin receptor DCC/DCC protein, expressed by HEK293, with C-His, C-10*His labeled tag. The total length of Netrin receptor DCC/DCC Protein, Human (HEK293, His) is 1066 a.a., with molecular weight of 125-150 kDa.

DCC, a critical receptor for netrin, plays a pivotal role in axon guidance by mediating axon attraction in neuronal growth cones during nervous system development upon ligand binding. Its intricate associations with UNC5 proteins may trigger signaling pathways leading to axon repulsion. Additionally, DCC acts as a dependence receptor, instigating apoptosis when not bound to the netrin ligand. Beyond its role in neural development, DCC is implicated as a tumor suppressor gene. The receptor engages in diverse protein interactions, including the cytoplasmic parts of UNC5A, UNC5B, UNC5C, and possibly UNC5D, as well as with DSCAM, PTK2/FAK1, MAPK1, NTN1, MYO10, CBLN4, SIAH1, and SIAH2. These interactions underscore the multifaceted functions of DCC in orchestrating axon guidance, apoptosis, and potential tumor suppression.

Immobilized Recombinant Human DCC at 1 μg/ml (100 μl/well) can bind Biotinylated Recombinant Human Netrin-1 Protein. The ED₅₀ for this effect is 85.29 ng/mL.

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  Immobilized Recombinant Human DCC at 1 μg/ml (100 μl/well) can bind Biotinylated Recombinant Human Netrin-1 Protein. The ED₅₀ for this effect is 85.29 ng/mL.

Human

HEK293

C-His;C-10*His

P43146-1 (F32-N1097)

1630  [NCBI]

FQIKAFTALRFLSEPSDAVTMRGGNVLLDCSAESDRGVPVIKWKKDGIHLALGMDERKQQLSNGSLLIQNILHSRHHKPDEGLYQCEASLGDSGSIISRTAKVAVAGPLRFLSQTESVTAFMGDTVLLKCEVIGEPMPTIHWQKNQQDLTPIPGDSRVVVLPSGALQISRLQPGDIGIYRCSARNPASSRTGNEAEVRILSDPGLHRQLYFLQRPSNVVAIEGKDAVLECCVSGYPPPSFTWLRGEEVIQLRSKKYSLLGGSNLLISNVTDDDSGMYTCVVTYKNENISASAELTVLVPPWFLNHPSNLYAYESMDIEFECTVSGKPVPTVNWMKNGDVVIPSDYFQIVGGSNLRILGVVKSDEGFYQCVAENEAGNAQTSAQLIVPKPAIPSSSVLPSAPRDVVPVLVSSRFVRLSWRPPAEAKGNIQTFTVFFSREGDNRERALNTTQPGSLQLTVGNLKPEAMYTFRVVAYNEWGPGESSQPIKVATQPELQVPGPVENLQAVSTSPTSILITWEPPAYANGPVQGYRLFCTEVSTGKEQNIEVDGLSYKLEGLKKFTEYSLRFLAYNRYGPGVSTDDITVVTLSDVPSAPPQNVSLEVVNSRSIKVSWLPPPSGTQNGFITGYKIRHRKTTRRGEMETLEPNNLWYLFTGLEKGSQYSFQVSAMTVNGTGPPSNWYTAETPENDLDESQVPDQPSSLHVRPQTNCIIMSWTPPLNPNIVVRGYIIGYGVGSPYAETVRVDSKQRYYSIERLESSSHYVISLKAFNNAGEGVPLYESATTRSITDPTDPVDYYPLLDDFPTSVPDLSTPMLPPVGVQAVALTHDAVRVSWADNSVPKNQKTSEVRLYTVRWRTSFSASAKYKSEDTTSLSYTATGLKPNTMYEFSVMVTKNRRSSTWSMTAHATTYEAAPTSAPKDLTVITREGKPRAVIVSWQPPLEANGKITAYILFYTLDKNIPIDDWIMETISGDRLTHQIMDLNLDTMYYFRIQARNSKGVGPLSDPILFRTLKVEHPDKMANDQGRHGDGGYWPVDTNLIDRSTLNEPPIGQMHPPHGSVTPQKNSN

125-150 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.