NEU2 Protein, Human (HEK293, His)
NEU2 protein acts as an exo-α sialidase and selectively hydrolyzes terminal sialic acids in glycolipids, glycoproteins, and oligosaccharides. It shows specificity for α-(2->3)-sialylated GD1a and GT1B gangliosides, preferring α-(2->3) over α-(2->8)-sialylated GD1b. NEU2 Protein, Human (HEK293, His) is the recombinant human-derived NEU2 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
NEU2 protein acts as an exo-α sialidase and selectively hydrolyzes terminal sialic acids in glycolipids, glycoproteins, and oligosaccharides. It shows specificity for α-(2->3)-sialylated GD1a and GT1B gangliosides, preferring α-(2->3) over α-(2->8)-sialylated GD1b. NEU2 Protein, Human (HEK293, His) is the recombinant human-derived NEU2 protein, expressed by HEK293 , with C-His labeled tag.
Background
The NEU2 Protein functions as an exo-alpha-sialidase, catalyzing the hydrolytic cleavage of terminal sialic acid in the catabolism of glycolipids, glycoproteins, and oligosaccharides. It demonstrates specificity for alpha-(2->3)-sialylated GD1a and GT1B gangliosides, showing preference over alpha-(2->8)-sialylated GD1b. While it hydrolyzes monomeric GM1 ganglioside, it lacks activity toward the miscellar form. NEU2 exhibits lower sialidase activity for glycoproteins like fetuin and TF/transferrin carrying a mixture of alpha-(2->3) and alpha-(2->6)-sialyl linkages. It cleaves milk oligosaccharide alpha-(2->3)-sialyllactose but remains inactive toward the alpha-(2->6)-sialyllactose isomer and colominic acid, a homomer of alpha-(2->8)-linked Neu5Ac residues.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q9Y3R4 (M1-Q380)
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Molecular Construction
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N-term
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NEU2 (M1-Q380)
Accession # Q9Y3R4 -
His
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C-term
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Protein Length
Full Length
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Synonyms
NEU2; Sialidase 2 (Cytosolic Sialidase); Neuraminidase 2; Cytosolic Sialidase; N-Acetyl-Alpha-Neuraminidase 2; Sialidase-2; SIAL2; Neuraminidase 2 (Cytosolic Sialidase)
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AA Sequence
MASLPVLQKESVFQSGAHAYRIPALLYLPGQQSLLAFAEQRASKKDEHAELIVLRRGDYDAPTHQVQWQAQEVVAQARLDGHRSMNPCPLYDAQTGTLFLFFIAIPGQVTEQQQLQTRANVTRLCQVTSTDHGRTWSSPRDLTDAAIGPAYREWSTFAVGPGHCLQLHDRARSLVVPAYAYRKLHPIQRPIPSAFCFLSHDHGRTWARGHFVAQDTLECQVAEVETGEQRVVTLNARSHLRARVQAQSTNDGLDFQESQLVKKLVEPPPQGCQGSVISFPSPRSGPGSPAQWLLYTHPTHSWQRADLGAYLNPRPPAPEAWSEPVLLAKGSCAYSDLQSMGTGPDGSPLFGCLYEANDYEEIVFLMFTLKQAFPAEYLPQ
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Predicted Molecular Mass
43.7 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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