NIT2 Protein, Arabidopsis thaliana
The NIT2 protein has the extraordinary ability to catalyze the conversion of 3-indole-acetonitrile into 3-indole-acetic acid, an important plant hormone involved in many physiological processes. This transition is significant because it represents a key step in the biosynthesis of indole-3-acetic acid, a key regulator of plant growth and development. NIT2 Protein, Arabidopsis thaliana is the recombinant NIT2 protein, expressed by E. coli , with tag free.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Biological Activity
Description
The NIT2 protein has the extraordinary ability to catalyze the conversion of 3-indole-acetonitrile into 3-indole-acetic acid, an important plant hormone involved in many physiological processes. This transition is significant because it represents a key step in the biosynthesis of indole-3-acetic acid, a key regulator of plant growth and development. NIT2 Protein, Arabidopsis thaliana is the recombinant NIT2 protein, expressed by E. coli , with tag free.
Background
NIT2 protein possesses the remarkable ability to catalyze the conversion of indole-3-acetonitrile into indole-3-acetic acid, which is a vital plant hormone involved in numerous physiological processes. This transformation is significant as it represents a key step in the biosynthesis of indole-3-acetic acid, a crucial regulator of plant growth and development. NIT2's enzymatic activity contributes to the homeostasis of indole-3-acetic acid levels, which in turn influences plant organogenesis, cell elongation, root development, and responses to environmental stimuli. Understanding the role and regulation of NIT2 protein in this metabolic pathway can provide insights into plant hormone signaling and potentially be harnessed for agricultural applications, such as enhancing crop yield or stress tolerance.
Technical Parameters
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Species Others
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Source E. coli
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Tag Tag Free
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Accession
P32962 (M1-K339)
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Gene ID823555
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Molecular Construction
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N-term
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NIT2 (M1-K339)
Accession # P32962 -
C-term
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Protein Length
Full Length
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Synonyms
NIT2; Epididymis Secretory Sperm Binding Protein Li 8a; Nitrilase Family Member 2; Nitrilase Family, Member 2; Omega-Amidase NIT2; Nit Protein 2; Nitrilase Homolog 2; HEL-S-8a
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AA Sequence
MSTSENTPFNGVASSTIVRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGLGVGVHNEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALFFSPQGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCLRKDFPDHPDYLFTDWYDDKEPDSIVSQGGSVIISPLGQVLAGPNFESEGLITADLDLGDVARAKLYFDSVGHYSRPDVLHLTVNEHPKKPVTFISKVEKAEDDSNK
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)