NIT2 Protein, Arabidopsis thaliana

The NIT2 protein has the extraordinary ability to catalyze the conversion of 3-indole-acetonitrile into 3-indole-acetic acid, an important plant hormone involved in many physiological processes. This transition is significant because it represents a key step in the biosynthesis of indole-3-acetic acid, a key regulator of plant growth and development. NIT2 Protein, Arabidopsis thaliana is the recombinant NIT2 protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Others
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

The NIT2 protein has the extraordinary ability to catalyze the conversion of 3-indole-acetonitrile into 3-indole-acetic acid, an important plant hormone involved in many physiological processes. This transition is significant because it represents a key step in the biosynthesis of indole-3-acetic acid, a key regulator of plant growth and development. NIT2 Protein, Arabidopsis thaliana is the recombinant NIT2 protein, expressed by E. coli , with tag free.

Background

NIT2 protein possesses the remarkable ability to catalyze the conversion of indole-3-acetonitrile into indole-3-acetic acid, which is a vital plant hormone involved in numerous physiological processes. This transformation is significant as it represents a key step in the biosynthesis of indole-3-acetic acid, a crucial regulator of plant growth and development. NIT2's enzymatic activity contributes to the homeostasis of indole-3-acetic acid levels, which in turn influences plant organogenesis, cell elongation, root development, and responses to environmental stimuli. Understanding the role and regulation of NIT2 protein in this metabolic pathway can provide insights into plant hormone signaling and potentially be harnessed for agricultural applications, such as enhancing crop yield or stress tolerance.

Technical Parameters

  • Species Others
  • Source E. coli
  • Tag Tag Free
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • NIT2 (M1-K339)
      Accession # P32962
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    NIT2; Epididymis Secretory Sperm Binding Protein Li 8a; Nitrilase Family Member 2; Nitrilase Family, Member 2; Omega-Amidase NIT2; Nit Protein 2; Nitrilase Homolog 2; HEL-S-8a

  • AA Sequence

    MSTSENTPFNGVASSTIVRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGLGVGVHNEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALFFSPQGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCLRKDFPDHPDYLFTDWYDDKEPDSIVSQGGSVIISPLGQVLAGPNFESEGLITADLDLGDVARAKLYFDSVGHYSRPDVLHLTVNEHPKKPVTFISKVEKAEDDSNK

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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