Notch 4 Protein, Human (sf9, His)
NOTCH4 protein is a receptor for the ligands JAG1, JAG2 and DLL1 and is critical for cell fate decisions. Upon ligand activation, NICD forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes in the cleavage site enhancer. Notch 4 Protein, Human (sf9, His) is the recombinant human-derived NOTCH4 protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
NOTCH4 protein is a receptor for the ligands JAG1, JAG2 and DLL1 and is critical for cell fate decisions. Upon ligand activation, NICD forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes in the cleavage site enhancer. Notch 4 Protein, Human (sf9, His) is the recombinant human-derived NOTCH4 protein, expressed by Sf9 insect cells , with C-His labeled tag.
Background
NOTCH4 protein functions as a receptor for membrane-bound ligands, including Jagged1, Jagged2, and Delta1, crucial for the regulation of cell-fate determination. Upon ligand activation, the released notch intracellular domain (NICD) forms a transcriptional activator complex with RBPJ/RBPSUH, leading to the activation of genes within the enhancer of split locus. Its role extends to influencing differentiation, proliferation, and apoptotic programs, potentially contributing to the regulation of branching morphogenesis in the developing vascular system. Structurally, NOTCH4 exists as a heterodimer, consisting of a C-terminal fragment (N(TM)) and a N-terminal fragment (N(EC)), likely connected by disulfide bonds. The protein interacts with transcriptional coactivators MAML1, MAML2, and MAML3, facilitating their role in modulating downstream transcriptional processes.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag C-His
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Accession
AAH63815.1 (R24-F637)
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Molecular Construction
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N-term
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NOTCH4 (R24-F637)
Accession # AAH63815.1 -
His
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
NOTCH4; Notch (Drosophila) Homolog 4; Prev. INT3; Notch Homolog 4; Neurogenic Locus Notch Homolog Protein 4; NOTCH4 Protein; Notch 4; HNotch4; Notch Receptor 4; Notch Homolog 4 (Drosophila)
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AA Sequence
RGLLCGSFPEPCANGGTCLSLSLGQGTCQCAPGFLGETCQFPDPCQNAQLCQNGGSCQALLPAPLGLPSSPSPLTPSFLCTCLPGFTGERCQAKLEDPCPPSFCSKRGRCHIQASGRPQCSCMPGWTGEQCQLRDFCSANPCVNGGVCLATYPQIQCHCPPGFEGHACERDVNECFQDPGPCPKGTSCHNTLGSFQCLCPVGQEGPRCELRAGPCPPRGCSNGGTCQLMPEKDSTFHLCLCPPGFIGPDCEVNPDNCVSHQCQNGGTCQDGLDTYTCLCPETWTGWDCSEDVDECETQGPPHCRNGGTCQNSAGSFHCVCVSGWGGTSCEENLDDCIAATCAPGSTCIDRVGSFSCLCPPGRTGLLCHLEDMCLSQPCHGDAQCSTNPLTGSTLCLCQPGYSGPTCHQDLDECLMAQQGPSPCEHGGSCLNTPGSFNCLCPPGYTGSRCEADHNECLSQPCHPGSTCLDLLATFHCLCPPGLEGQLCEVETNECASAPCLNHADCHDLLNGFQCICLPGFSGTRCEEDIDECRSSPCANGGQCQDQPGAFHCKCLPGFEGPRCQTEVDECLSDPCPVGASCLDLPGAFFCLCPSGFTGSQGRHWKELAEYFIPF
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Predicted Molecular Mass
66.1 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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