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NPR1/NPRA Protein, Human (HEK293, His)

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Based on 1 publication(s) in Google Scholar

NPR1 Protein, a receptor for atrial natriuretic peptide NPPA/ANP and brain natriuretic peptide NPPB/BNP, initiates signaling cascades with guanylate cyclase activity upon ligand binding. Essential vasoactive hormones, these peptides regulate cardiovascular homeostasis. NPR1 significantly contributes to vascular tone and fluid balance regulation in the cardiovascular system through its role as a receptor for these natriuretic peptides. NPR1/NPRA Protein, Human (HEK293, His) is the recombinant human-derived NPR1 protein, expressed by HEK293 , with C-His labeled tag.

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  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • NPR1/NPRA Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

NPR1 Protein, a receptor for atrial natriuretic peptide NPPA/ANP and brain natriuretic peptide NPPB/BNP, initiates signaling cascades with guanylate cyclase activity upon ligand binding. Essential vasoactive hormones, these peptides regulate cardiovascular homeostasis. NPR1 significantly contributes to vascular tone and fluid balance regulation in the cardiovascular system through its role as a receptor for these natriuretic peptides. NPR1/NPRA Protein, Human (HEK293, His) is the recombinant human-derived NPR1 protein, expressed by HEK293 , with C-His labeled tag.

Background

The NPR1 Protein serves as a receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP, essential vasoactive hormones that play a pivotal role in cardiovascular homeostasis. Upon binding to these ligands, NPR1 exhibits guanylate cyclase activity, initiating signaling cascades crucial for maintaining cardiovascular balance. Through its role as a receptor for these natriuretic peptides, NPR1 contributes significantly to the regulation of vascular tone and fluid balance in the cardiovascular system.

Verified Bioactivity

Immobilized Anti-NPR1 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human NPR1, His Tag with the EC50 of 6.5-7.2 ng/ml determined by ELISA.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-8*His
  • Accession

    P16066 (G33-E473)

  • Gene ID
    4881
  • Molecular Construction
    • N-term
    • NPR1 (G33-E473)
      Accession # P16066
    • 8*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    NPR1; ANPR-A; Prev. ANPRA; ANP-A; Prev. NPRA; GC-A; Guanylate Cyclase A; Natriuretic Peptide Receptor A/Guanylate Cyclase A (Atrionatriuretic Peptide Receptor A); GUCY2A; Natriuretic Peptide A Type Receptor; ANPa; Testicular Tissue Protein Li 20; Atrial N

  • AA Sequence

    GNLTVAVVLPLANTSYPWSWARVGPAVELALAQVKARPDLLPGWTVRTVLGSSENALGVCSDTAAPLAAVDLKWEHNPAVFLGPGCVYAAAPVGRFTAHWRVPLLTAGAPALGFGVKDEYALTTRAGPSYAKLGDFVAALHRRLGWERQALMLYAYRPGDEEHCFFLVEGLFMRVRDRLNITVDHLEFAEDDLSHYTRLLRTMPRKGRVIYICSSPDAFRTLMLLALEAGLCGEDYVFFHLDIFGQSLQGGQGPAPRRPWERGDGQDVSARQAFQAAKIITYKDPDNPEYLEFLKQLKHLAYEQFNFTMEDGLVNTIPASFHDGLLLYIQAVTETLAHGGTVTDGENITQRMWNRSFQGVTGYLKIDSSGDRETDFSLWDMDPENGAFRVVLNYNGTSQELVAVSGRKLNWPLGYPPPDIPKCGFDNEDPACNQDHLSTLE

  • Predicted Molecular Mass

    50.5 kDa

  • Molecular Weight

    Approximately 60-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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