NSMCE1 Protein, Human (His)
NSMCE1 Protein, Human (His) is the recombinant human-derived NSMCE1, expressed by E. coli , with His labeled tag. ,
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
NSMCE1 Protein, Human (His) is the recombinant human-derived NSMCE1, expressed by E. coli , with His labeled tag. ,
NSMCE1 is a RING-type zinc finger-containing E3 ubiquitin ligase that forms a complex with melanoma antigen protein (MAGE) to catalyze the direct transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to specific substrates. Within the MAGE-RING ubiquitin ligase complex, MAGE likely stimulates and specifies ubiquitin ligase activity by recruiting and/or stabilizing the E2 enzyme at the E3:substrate complex. It plays roles in genome integrity maintenance, DNA damage response, and DNA repair. NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of the SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair. Additionally, the MAGEF1-NSMCE1 ubiquitin ligase promotes proteasomal degradation of MMS19, a core component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Downregulation of MMS19 impairs the activity of DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1, and POLD1, which require iron-sulfur clusters as cofactors.
Technical Parameters
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Species Human
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Source E. coli
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Tag His
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Accession
Q8WV22 (Q2-H266)
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Gene ID197370
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Protein Length
Partial
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Synonyms
NSMCE1; NSE1; NSE1 Component Of SMC5/6 Complex; Non-SMC Element 1 Homolog; NSE1 Homolog, SMC5-SMC6 Complex Component; Non-SMC Element 1 Homolog (S. Cerevisiae); Non-Structural Maintenance Of Chromosomes Element 1 Homolog
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AA Sequence
QGSTRRMGVMTDVHRRFLQLLMTHGVLEEWDVKRLQTHCYKVHDRNATVDKLEDFINNINSVLESLYIEIKRGVTEDDGRPIYALVNLATTSISKMATDFAENELDLFRKALELIIDSETGFASSTNILNLVDQLKGKKMRKKEAEQVLQKFVQNKWLIEKEGEFTLHGRAILEMEQYIRETYPDAVKICNICHSLLIQGQSCETCGIRMHLPCVAKYFQSNAEPRCPHCNDYWPHEIPKVFDPEKERESGVLKSNKKSLRSRQH
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Predicted Molecular Mass
32.9 kDa
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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