SARS-CoV-2 NSP3 Protein (338a.a)
The multifunctional PL-PRO protein plays a critical role in viral RNA transcription and replication, providing the necessary proteases for multiprotein cleavage. It strategically blocks host translation by binding to the open head conformation of the 40S subunit, impeding ribosomal mRNA entry into the tunnel. NSP3 Protein, SARS-CoV-2 (E746-K1083) is the recombinant virus-derived NSP3, expressed by E. coli, with tag-free.
- Species: Virus
- Source: E. coli
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The multifunctional PL-PRO protein plays a critical role in viral RNA transcription and replication, providing the necessary proteases for multiprotein cleavage. It strategically blocks host translation by binding to the open head conformation of the 40S subunit, impeding ribosomal mRNA entry into the tunnel. NSP3 Protein, SARS-CoV-2 (E746-K1083) is the recombinant virus-derived NSP3, expressed by E. coli, with tag-free.
Background
nsp3 (papain-like protease) is a key functional protein in the viral replicase polyprotein, mainly responsible for the cleavage of the N-terminus of the replicase polyprotein; it participates in the assembly of virus-induced cytoplasmic double-membrane vesicles (DMVs) together with nsp4 (DMVs are necessary for viral replication) and plays a role in its formation and maintenance (DMVs are formed by nsp3 and nsp4, and nsp6 is involved in the connection of the endoplasmic reticulum membrane and the binding to lipid droplets). At the same time, nsp3 inhibits the induction of type I interferon by blocking the phosphorylation, dimerization and nuclear translocation of host IRF3, and can also inhibit NF-κB signaling, thereby antagonizing innate immunity. In addition, it has deubiquitination/de-ISG activity, can process Lys-48 and Lys-63-linked polyubiquitin chains in cellular substrates, preferentially cuts ISG15 from the antiviral protein IFIH1 (MDA5) (has no effect on RIGI), and participates in the host ADP-ribosylation process.
Technical Parameters
-
Species Virus
-
Source E. coli
-
Tag Tag Free
-
Accession
P0DTC1-1 (E1564-K1901)
-
Gene ID43740578
-
Protein Length
Partial
-
Synonyms
Replicase polyprotein 1a; ORF1a polyprotein; Severe acute respiratory syndrome coronavirus 2; 2019-nCoV; pp1a; Endonuclease; Hydrolase; Methyltransferase; Nuclease; Protease; RNA-binding; Thiol protease; Transferase
-
AA Sequence
EVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYK
-
Predicted Molecular Mass
38.3 kDa
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
