NTN1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
Netrin-1 protein is a key axon guidance regulator that guides commissural and peripheral motor axons in the central nervous system. Binding to DCC or UNC5 receptors determines axonal attraction or repulsion. NTN1 Protein, Human (HEK293, His) is the recombinant human-derived NTN1 protein, expressed by HEK293, with C-His labeled tag.
- Species: Human
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Netrin-1 protein is a key axon guidance regulator that guides commissural and peripheral motor axons in the central nervous system. Binding to DCC or UNC5 receptors determines axonal attraction or repulsion. NTN1 Protein, Human (HEK293, His) is the recombinant human-derived NTN1 protein, expressed by HEK293, with C-His labeled tag.
Background
Netrin-1, a crucial regulator of axonal guidance in both the central nervous system (CNS) and peripheral motor neurons, plays a pivotal role in directing commissural axons within the CNS and motor axons in the periphery. The association of Netrin-1 with either DCC or certain UNC5 receptors dictates whether axons are attracted or repelled. Specifically, binding to UNC5C induces the dissociation of UNC5C from polymerized TUBB3 in microtubules, leading to heightened microtubule dynamics and subsequent axon repulsion. Netrin-1 is essential for dorsal root ganglion axon projection toward the spinal cord and functions as a survival factor by preventing the initiation of apoptosis through its interactions with receptors. Furthermore, Netrin-1's involvement in tumorigenesis underscores its regulatory role in apoptosis. The protein binds to a repertoire of receptors, including DCC, UNC5A, UNC5B, UNC5C, UNC5D, and DSCAM, and exhibits interaction with APP, highlighting its multifaceted roles in cellular processes.
Verified Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human Netrin-1, His Tag at 5 μg/mL (100 μL/well) can bind Human Netrin receptor DCC, Fc Tag with a linear range of 5.0-20.0 ng/mL.
Technical Parameters
-
Species Human
-
Source HEK293
-
Tag C-His
-
Accession
O95631-1 (V22-A604)
-
Gene ID9423
-
Molecular Construction
-
N-term
-
NTN1 (V22-A604)
Accession # O95631-1 -
His
-
C-term
-
-
Protein Length
Full Length of Isoform-1
-
Synonyms
NTN1; NET1; Netrin 1; Epididymis Tissue Protein Li 131P; NTN1L; Netrin 1, Mouse, Homolog Of; Netrin-1; MRMV4
-
AA Sequence
VRGGPGLSMFAGQAAQPDPCSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPARYCVVSERGEERLRSCHLCNASDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQNEQEAVCTDSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYFYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGYYRDMGKPITHRKACKACDCHPVGAAGKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSVEEPEDCDSYCKASKGKLKINMKKYCKKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRRGDQSLWIRSRDIACKCPKIKPLKKYLLLGNAEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKKA
-
Predicted Molecular Mass
67.5 kDa
-
Molecular Weight
Approximately 80-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Glycosylation
Yes
-
Structure/Form
Monomer
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 with 0.3 M NaCl with 10% trehalose as protectant.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (239 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
