Nucleocapsid Protein NTD domain, Virus (His)

Nucleocapsid Protein NTD domain, Virus (His) is the N-terminal RNA-binding domain (NTD) of nucleocapsid protein in coronaviruses. Nucleocapsid Protein NTD domain has highly conserved residues in the centre, which is identified as the RNA-binding site^[1].

Coronaviruses are enveloped viruses with a positive-sense RNA genomeand and has a nucleocapsid of helical symmetry. The Nucleocapsid Protein localize to the cytoplasm and the nucleolus in both virus-infected primary cells and in cells transfected with plasmids that express N protein^[1].
Nucleocapsid Protein is a basic protein and is abundant in coronaviruses, which has nonspecific binding activity toward nucleic acids, including ssRNA, ssDNA, and dsDNA. Nucleocapsid Protein is an essential RNA-binding viral protein in human coronavirus (CoV)-infected cells, is required for the replication and transcription of viral RNA. Nucleocapsid Protein NTD domain has highly conserved residues in the centre, which is identified as the RNA-binding site^[2].

Immobilized Virus Nucleocapsid at 10 μg/mL (100 μL/well) can bind Anti-Nucleocapsid Antibody. The ED₅₀ for this effect is 2.927 μg/mL.

Virus

E. coli

N-6*His

QHD43423.2 (M1-Y109)

43740575  [NCBI]

MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWY

Approximately 15.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filter solution of PBS, 2 M Urea, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Sander van Boheemen, et al. Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans. mBio. 2012 Nov 20;3(6):e00473-12.  [Content Brief]

  [2]. Chung-ke Chang, et al. Structure-based virtual screening and experimental validation of the discovery of inhibitors targeted towards the human coronavirus nucleocapsid protein. Mol Biosyst. 2016 Jan;12(1):59-66.  [Content Brief]