Nucleocapsid Protein NTD domain, Virus (His)
Based on 1 Customer Validation
Nucleocapsid Protein NTD domain, Virus (His) is the N-terminal RNA-binding domain (NTD) of nucleocapsid protein in coronaviruses. Nucleocapsid Protein NTD domain has highly conserved residues in the centre, which is identified as the RNA-binding site.
- Species: Virus
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Nucleocapsid Protein NTD domain, Virus (His) is the N-terminal RNA-binding domain (NTD) of nucleocapsid protein in coronaviruses. Nucleocapsid Protein NTD domain has highly conserved residues in the centre, which is identified as the RNA-binding site[1].
Background
Coronaviruses are enveloped viruses with a positive-sense RNA genomeand and has a nucleocapsid of helical symmetry. The Nucleocapsid Protein localize to the cytoplasm and the nucleolus in both virus-infected primary cells and in cells transfected with plasmids that express N protein[1].Nucleocapsid Protein is a basic protein and is abundant in coronaviruses, which has nonspecific binding activity toward nucleic acids, including ssRNA, ssDNA, and dsDNA. Nucleocapsid Protein is an essential RNA-binding viral protein in human coronavirus (CoV)-infected cells, is required for the replication and transcription of viral RNA. Nucleocapsid Protein NTD domain has highly conserved residues in the centre, which is identified as the RNA-binding site[2].
Verified Bioactivity
Immobilized Virus Nucleocapsid at 10 μg/mL (100 μL/well) can bind Anti-Nucleocapsid Antibody. The ED50 for this effect is 2.927 μg/mL.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - ELISA
Bioactivity - ELISA
Technical Parameters
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Species Virus
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Source E. coli
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Tag N-6*His
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Accession
QHD43423.2 (M1-Y109)
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Gene ID43740575 [NCBI]
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Molecular Construction
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N-term
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6*His
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Nucleocapsid NTD (M1-Y109)
Accession # QHD43423.2 -
C-term
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Protein Length
Partial
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Synonyms
2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein
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AA Sequence
MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWY
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Molecular Weight
Approximately 16 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of PBS, 2 M Urea, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (261 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Sander van Boheemen, et al. Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans. mBio. 2012 Nov 20;3(6):e00473-12. [Content Brief]
[2]. Chung-ke Chang, et al. Structure-based virtual screening and experimental validation of the discovery of inhibitors targeted towards the human coronavirus nucleocapsid protein. Mol Biosyst. 2016 Jan;12(1):59-66. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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