OBCAM/OPCML Protein, Mouse (HEK293, His)

OBCAM/OPCML Protein uniquely binds opioids, especially in the presence of acidic lipids, indicating a potential role in cellular interactions and signaling related to opioid binding. This dual affinity suggests involvement in cell-contact processes where opioids and acidic lipids are present, highlighting its significance in modulating cellular responses to external stimuli and contributing to opioid-related signaling pathways. OBCAM/OPCML Protein, Mouse (HEK293, His) is the recombinant mouse-derived OBCAM/OPCML protein, expressed by HEK293 , with C-His labeled tag. The total length of OBCAM/OPCML Protein, Mouse (HEK293, His) is 284 a.a., with molecular weight of ~44-55 kDa.

The OBCAM/OPCML Protein demonstrates a unique ability to bind opioids, particularly in the presence of acidic lipids, suggesting a potential role in cellular interactions and signaling related to opioid binding. This characteristic implies that OBCAM/OPCML may be involved in cell-contact processes where opioids and acidic lipids are present. The protein's dual affinity for opioids and acidic lipids underscores its potential significance in modulating cellular responses to external stimuli and highlights its potential contribution to opioid-related signaling pathways.

Measured by its binding ability in a functional ELISA. Immobilized mouse OBCAM at 0.5 μg/mL (100 μL/well) can bind biotinylated human LSAMP. The ED₅₀ for this effect is 0.9972 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized mouse OBCAM at 0.5 μg/mL (100 μL/well) can bind biotinylated human LSAMP .The ED₅₀ for this effect is 0.9972 μg/mL.

Mouse

HEK293

C-His

Q6DFY2 (T30-V313)

330908  [NCBI]

TFPKAMDNVTVRQGESATLRCTIDDRVTRVAWLNRSTILYAGNDKWSIDPRVIILVNTPTQYSIMIQNVDVYDEGPYTCSVQTDNHPKTSRVHLIVQVPPQIMNISSDITVNEGSSVTLLCLAIGRPEPTVTWRHLSVKGQGFVSEDEYLEISDIKRDQSGEYECSALNDVAAPDVRKVKITVNYPPYISKAKNTGVSVGQKGILSCEASAVPMAEFQWFKEDTRLATGLDGVRIENKGRISTLTFFNVSEKDYGNYTCVATNKLGNTNASITLYGPGAVIDGV

Approximately 44-55 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.