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  3. ODC1 Protein, Human (HEK293, His-T7)

ODC1 Protein, Human (HEK293, His-T7)

Cat. No.: HY-P71109
COA Handling Instructions

The ODC1 protein is an important enzyme that catalyzes the epimerization of NAD(P)HX, a damaged NAD(P)H derivative. This activity is critical for subsequent repair by S-specific NAD(P)H hydrate dehydratase. ODC1 Protein, Human (HEK293, His-T7) is the recombinant human-derived ODC1 protein, expressed by E. coli , with N-T7, C-6*His labeled tag. The total length of ODC1 Protein, Human (HEK293, His-T7) is 461 a.a., with molecular weight of 25 & 58 kDa, respectively.

For research use only. We do not sell to patients.

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Description

The ODC1 protein is an important enzyme that catalyzes the epimerization of NAD(P)HX, a damaged NAD(P)H derivative. This activity is critical for subsequent repair by S-specific NAD(P)H hydrate dehydratase. ODC1 Protein, Human (HEK293, His-T7) is the recombinant human-derived ODC1 protein, expressed by E. coli , with N-T7, C-6*His labeled tag. The total length of ODC1 Protein, Human (HEK293, His-T7) is 461 a.a., with molecular weight of 25 & 58 kDa, respectively.

Background

APOA1BP protein is a key enzyme responsible for the epimerization of the S- and R-forms of NAD(P)HX, a damaged derivative of NAD(P)H resulting from enzymatic or heat-dependent hydration. This crucial catalytic activity is a prerequisite for the subsequent repair of both epimers of NAD(P)HX by the S-specific NAD(P)H-hydrate dehydratase. Additionally, APOA1BP exhibits a regulatory role in angiogenesis by facilitating cholesterol efflux from endothelial cells to high-density lipoprotein (HDL). This dual functionality underscores its significance in cellular metabolism and vascular processes, positioning APOA1BP as a key player in maintaining cellular homeostasis and influencing angiogenic pathways.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

E. coli

Tag

N-T7;C-6*His

Accession

P11926 (M1-V461)

Gene ID
Molecular Construction
N-term
T7
ODC1 (M1-V461)
Accession # P11926
6*His
C-term
Synonyms
Ornithine decarboxylase; ODC1;
AA Sequence

MNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGDILKKHLRWLKALPRVTPFYAVKCNDSKAIVKTLAATGTGFDCASKTEIQLVQSLGVPPERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHPKAKLVLRIATDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFHVGSGCTDPETFVQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLKFEEITGVINPALDKYFPSDSGVRIIAEPGRYYVASAFTLAVNIIAKKIVLKEQTGSDDEDESSEQTFMYYVNDGVYGSFNCILYDHAHVKPLLQKRPKPDEKYYSSSIWGPTCDGLDRIVERCDLPEMHVGDWMLFENMGAYTVAAASTFNGFQRPTIYYVMSGPAWQLMQQFQNPDFPPEVEEQDASTLPVSCAWESGMKRHRAACASASINV

Molecular Weight

25 &58 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 8% Trehalose, 2% Mannitol, 4 mM TCEP, 0.05% Tween 80, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

ODC1 Protein, Human (HEK293, His-T7) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
ODC1 Protein, Human (HEK293, His-T7)
Cat. No.:
HY-P71109
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