Optineurin Protein, Human (sf9, His)

Optineurin proteins are critical for maintaining the Golgi complex, coordinating membrane trafficking, and regulating exocytosis through interactions with myosin VI and Rab8. It links myosin VI to the Golgi apparatus to form a ribbon and activates the innate immune response during viral infection by recruiting TBK1 to the Golgi apparatus. Optineurin Protein, Human (sf9, His) is the recombinant human-derived Optineurin protein, expressed by Sf9 insect cells , with C-His labeled tag.

Optineurin protein plays a pivotal role in maintaining the Golgi complex, orchestrating membrane trafficking, and regulating exocytosis through its interactions with myosin VI and Rab8. Its linkage of myosin VI to the Golgi complex is integral to Golgi ribbon formation. Furthermore, Optineurin contributes to the activation of the innate immune response during viral infection by recruiting TBK1 to the Golgi apparatus, facilitating its trans-phosphorylation upon stimulation by RLR or TLR3. Activated TBK1, in turn, phosphorylates downstream partner IRF3, leading to the production of IFN-beta/IFNB1. In a neuroprotective role, particularly in the eye and optic nerve, Optineurin regulates membrane trafficking and cellular morphogenesis within a complex involving Rab8 and huntingtin. Additionally, Optineurin serves as an autophagy receptor, interacting with cargo destined for degradation, including ubiquitin-coated bacteria (xenophagy). Notably, it may be targeted by various viruses to inhibit the innate immune response, indicating its multifaceted functions in cellular homeostasis and defense mechanisms.

Human

Sf9 insect cells

C-His

Q96CV9 (M1-I577)

10133

Full Length

MSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPEELLQQMKELLTENHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEIQSKEAKERLMALSHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQEKDQLRTQVVRLQAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII

68.0 kDa

≥ 85%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.