OTULIN Protein, Human (His, GST)
OTULIN Protein, Human (His, GST) is the recombinant human-derived OTULIN, expressed by E. coli , with His, GST labeled tag. ,
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
OTULIN Protein, Human (His, GST) is the recombinant human-derived OTULIN, expressed by E. coli , with His, GST labeled tag. ,
Background
OTULIN is a deubiquitinase that specifically cleaves linear ('Met-1'-linked) polyubiquitin chains and plays a critical role in angiogenesis, innate immunity, and the regulation of Wnt and NF-kappa-B signaling pathways. It supports angiogenesis, craniofacial, and neuronal development by modulating canonical Wnt signaling alongside the LUBAC complex. As a negative regulator of NF-kappa-B, OTULIN controls LUBAC activity by removing its autoubiquitination, thus maintaining its function. It also acts as a key suppressor of inflammation and autoimmunity by restricting excessive cytokine secretion and linear ubiquitination. During NOD2 stimulation, OTULIN limits linear ubiquitin chain formation on LUBAC to modulate innate immune responses. by similar: OTULIN primarily functions in maintaining LUBAC complex homeostasis.
Technical Parameters
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Species Human
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Source E. coli
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Tag His;GST
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Accession
Q96BN8 (M1-L352)
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Gene ID90268
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Protein Length
Full Length
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Synonyms
OTULIN; Ubiquitin Thioesterase Gumby; Prev. FAM105B; AIPDSA; Ubiquitin Thioesterase Otulin; IMD107; OTU Domain-Containing Deubiquitinase With Linear Linkage Specificity; Gumby; Family With Sequence Similarity 105, Member B; AIPDS; Deubiquitinating Enzyme Otulin; GUM; OTU Deubiquitinase With Linear Linkage Specificity; FLJ34884
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AA Sequence
MSRGTMPQPEAWPGASCAETPAREAAATARDGGKAAASGQPRPEMQCPAEHEEDMYRAADEIEKEKELLIHERGASEPRLSVAPEMDIMDYCKKEWRGNTQKATCMKMGYEEVSQKFTSIRRVRGDNYCALRATLFQAMSQAVGLPPWLQDPELMLLPEKLISKYNWIKQWKLGLKFDGKNEDLVDKIKESLTLLRKKWAGLAEMRTAEARQIACDELFTNEAEEYSLYEAVKFLMLNRAIELYNDKEKGKEVPFFSVLLFARDTSNDPGQLLRNHLNQVGHTGGLEQVEMFLLAYAVRHTIQVYRLSKYNTEEFITVYPTDPPKDWPVVTLIAEDDRHYNIPVRVCEETSL
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Predicted Molecular Mass
68 kDa
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Molecular Weight
Approximately 68 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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