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  4. P2ER3 Protein, Human (His)

P2ER3 Protein, Human (His) is the recombinant human-derived P2ER3, expressed by E.coli , with His abeled tag.

For research use only. We do not sell to patients.

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Description

P2ER3 Protein, Human (His) is the recombinant human-derived P2ER3, expressed by E.coli , with His abeled tag.
Background

P2ER3 is a receptor for prostaglandin E2 (PGE2), coupling to both the inhibition of adenylate cyclase via G(i) proteins and the elevation of intracellular calcium. It is essential for fever development in response to pyrogens such as IL1B, PGE2, and bacterial lipopolysaccharide (LPS). Additionally, P2ER3 potentiates PGE2-induced platelet aggregation, contributing to blood coagulation regulation. In the duodenum, it mediates increased HCO3(-) secretion upon mucosal acidification, protecting against acid-induced ulceration. By similarity, P2ER3 is not required for normal kidney function, urine volume, or osmolality.
Species

Human
Source

E. coli
Tag

C-6*His
Accession

P43115 (M1-A53)
Gene ID

5733
Molecular Construction
N-term
PTGER3 (M1-A53)
Accession # P43115
6*His
C-term
Protein Length

Partial
Synonyms
Prostaglandin E2 receptor EP3 subtype; PGE2-R; Prostanoid EP3 receptor; PTGER3; Homo sapiens; Human; PGE receptor EP3 subtype; G-protein coupled receptor; Receptor; Transducer
AA Sequence

MKETRGYGGDAPFCTRLNHSYTGMWAPERSAEARGNLTRPPGSGEDCGSVSVA

Predicted Molecular Mass
12.5 kDa
Purity

≥ 90%, as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

P2ER3 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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