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PDGF-DD Protein, Mouse (His-SUMO)

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Based on 1 Customer Validation

PDGF-DD protein is an important growth factor that controls embryonic development, cell proliferation, migration, survival and chemotaxis, and has a potent mitogenic effect on mesenchymal cells. It plays a key role in wound healing and has carcinogenic potential, contributing to tumor formation. PDGF-DD Protein, Mouse (His-SUMO) is the recombinant mouse-derived PDGF-DD protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for PDGF-DD Protein, Mouse (His-SUMO)
  • PDGF-DD Protein, Mouse (His-SUMO) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

PDGF-DD protein is an important growth factor that controls embryonic development, cell proliferation, migration, survival and chemotaxis, and has a potent mitogenic effect on mesenchymal cells. It plays a key role in wound healing and has carcinogenic potential, contributing to tumor formation. PDGF-DD Protein, Mouse (His-SUMO) is the recombinant mouse-derived PDGF-DD protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

Background

PDGF-DD Protein emerges as a crucial growth factor governing embryonic development, cell proliferation, migration, survival, and chemotaxis. Renowned for its potent mitogenic effects on mesenchymal cells, PDGF-DD assumes a pivotal role in wound healing and, intriguingly, harbors oncogenic potential capable of inducing tumor formation. Its influence extends to the intricate orchestration of events during angiogenesis, promoting macrophage recruitment, heightened interstitial pressure, and blood vessel maturation. PDGF-DD's involvement is notable in the initiation of processes leading to mesangial proliferative glomerulonephritis, marked by monocyte and macrophage influx, as well as extracellular matrix production. Structurally, PDGF-DD exists as a homodimer linked by disulfide bonds, engaging in interactions with PDGFRB homodimers and heterodimers formed by PDGFRA and PDGFRB, contributing to its versatile regulatory mechanisms.

Verified Bioactivity

Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 62.82 ng/mL, corresponding to a specific activity is 1.59×104 units/mg.

MCE Validation Data

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 62.82 ng/mL, corresponding to a specific activity is 1.59×104 units/mg.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag N-SUMO;N-6*His
  • Accession

    Q925I7-1 (T24-R370)

  • Gene ID
    71785  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His-SUMO
    • PDGF-DD (T24-R370)
      Accession # Q925I7-1
    • C-term
  • Protein Length

    Full Length of Isoform-1 Mature Protein

  • Synonyms

    PDGFD; Platelet-Derived Growth Factor D; Platelet Derived Growth Factor D; Iris-Expressed Growth Factor; SCDGF-B; SCDGFB; IEGF; PDGF-D; MSTP036; Spinal Cord Derived Growth Factor B; Spinal Cord-Derived Growth Factor B

  • AA Sequence

    TPQRASIKALRNANLRRDESNHLTDLYQREENIQVTSNGHVQSPRFPNSYPRNLLLTWWLRSQEKTRIQLSFDHQFGLEEAENDICRYDFVEVEEVSESSTVVRGRWCGHKEIPPRITSRTNQIKITFKSDDYFVAKPGFKIYYSFVEDFQPEAASETNWESVTSSFSGVSYHSPSITDPTLTADALDKTVAEFDTVEDLLKHFNPVSWQDDLENLYLDTPHYRGRSYHDRKSKVDLDRLNDDVKRYSCTPRNHSVNLREELKLTNAVFFPRCLLVQRCGGNCGCGTVNWKSCTCSSGKTVKKYHEVLKFEPGHFKRRGKAKNMALVDIQLDHHERCDCICSSRPPR

  • Predicted Molecular Mass

    56.2 kDa

  • Molecular Weight

    Approximately 66 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 62.82 ng/mL, corresponding to a specific activity is 1.59×104 units/mg.

  • PDGF-DD Protein, Mouse (His-SUMO) Product Image
    1/16
  • Experimental Validation Results for PDGF-DD Protein, Mouse (His-SUMO)
  • PDGF-DD Protein, Mouse (His-SUMO) Product Image