PDK1 Protein, Human (Active, sf9, His)
Based on 1 Customer Validation
PDK1 protein is a key kinase that controls glucose and fatty acid metabolism by phosphorylating pyruvate dehydrogenase subunits PDHA1 and PDHA2. This molecular switch inhibits pyruvate dehydrogenase, fine-tunes metabolite flux and downregulates aerobic respiration. PDK1, Human (Active, sf9, His) is the recombinant human-derived PDK1 protein, expressed by Sf9 insect cells, with N-6*His;N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
PDK1 protein is a key kinase that controls glucose and fatty acid metabolism by phosphorylating pyruvate dehydrogenase subunits PDHA1 and PDHA2. This molecular switch inhibits pyruvate dehydrogenase, fine-tunes metabolite flux and downregulates aerobic respiration. PDK1, Human (Active, sf9, His) is the recombinant human-derived PDK1 protein, expressed by Sf9 insect cells, with N-6*His;N-GST labeled tag.
Background
PDK1, a pivotal kinase, exerts significant control over glucose and fatty acid metabolism by phosphorylating the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This phosphorylation event acts as a molecular switch, inhibiting pyruvate dehydrogenase activity and finely tuning metabolite flux through the tricarboxylic acid cycle. Consequently, PDK1 down-regulates aerobic respiration and impedes the conversion of pyruvate to acetyl-coenzyme A. Beyond its metabolic functions, PDK1 emerges as a critical player in cellular responses to hypoxia, where it plays a key role in promoting cell proliferation under oxygen-deprived conditions. Moreover, PDK1 serves a protective role by shielding cells against apoptosis triggered by hypoxia and oxidative stress, showcasing its multifaceted contributions to cellular homeostasis and stress response pathways.
Verified Bioactivity
The activity was measured by ADP-Glo™ Kinase Assay. ATP: 10 µM, Substrate: 0.2 mg/mL PDKtide.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-6*His
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Accession
Q15118-1 (M1-A436)
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Gene ID5163
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Molecular Construction
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N-term
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6*His
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PDK1 (M1-A436)
Accession # Q15118-1 -
C-term
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Protein Length
Full Length
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Synonyms
PDK1; Mitochondrial Pyruvate Dehydrogenase, Lipoamide, Kinase Isoenzyme 1; Pyruvate Dehydrogenase Kinase 1; Pyruvate Dehydrogenase Kinase, Isozyme 1; [Pyruvate Dehydrogenase (Acetyl-Transferring)] Kinase Isozyme 1, Mitochondrial; Pyruvate Dehydrogenase Kinase Isoform 1; Pyruvate Dehydrogenase Kinase, Isoenzyme 1; Protein-Serine/Threonine Kinase; PDH Kinase 1; PDHK1; Pyruvate Dehydrogenase (Acetyl-Transferring) Kinase Isozyme 1, Mitochondrial
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Predicted Molecular Mass
66.5 kDa
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Molecular Weight
Approximately 65-70 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
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Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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