PITHD1 Protein, Human (Myc, His)
The PITHD1 protein is a key regulator of cell dynamics, specifically promoting megakaryocyte differentiation by upregulating RUNX1 expression. It does this by activating the proximal promoter of the RUNX1 gene and enhancing the translational activity of the internal ribosome entry site (IRES) element. PITHD1 Protein, Human (Myc, His) is the recombinant human-derived PITHD1 protein, expressed by E. coli , with N-His, C-Myc labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The PITHD1 protein is a key regulator of cell dynamics, specifically promoting megakaryocyte differentiation by upregulating RUNX1 expression. It does this by activating the proximal promoter of the RUNX1 gene and enhancing the translational activity of the internal ribosome entry site (IRES) element. PITHD1 Protein, Human (Myc, His) is the recombinant human-derived PITHD1 protein, expressed by E. coli , with N-His, C-Myc labeled tag.
Background
PITHD1 Protein emerges as a pivotal regulator in cellular dynamics, specifically promoting megakaryocyte differentiation by up-regulating the expression of RUNX1. Its regulatory influence extends to the modulation of RUNX1 expression, achieved through activation of the proximal promoter of the RUNX1 gene and enhancement of the translation activity of an internal ribosome entry site (IRES) element within the RUNX1 gene. The intricate interplay of PITHD1 in these processes highlights its crucial role in orchestrating megakaryocyte differentiation, underscoring its significance in hematopoiesis. Further exploration of the detailed mechanisms behind PITHD1's regulatory functions offers promising insights into its broader impact on cellular differentiation pathways.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His;C-Myc
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Accession
Q9GZP4-1 (M1-S211)
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Gene ID57095 [NCBI]
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Molecular Construction
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N-term
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10*His
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PITHD1 (M1-S211)
Accession # Q9GZP4-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
PITHD1; PITH (C-Terminal Proteasome-Interacting Domain Of Thioredoxin-Like) Domain Containing 1; Prev. C1orf128; TXNL1 C-Terminal Like; TXNL1CL; Chromosome 1 Open Reading Frame 128; HT014; PITH Domain Containing 1; PITH Domain-Containing Protein 1
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AA Sequence
MSHGHSHGGGGCRCAAEREEPPEQRGLAYGLYLRIDLERLQCLNESREGSGRGVFKPWEERTDRSKFVESDADEELLFNIPFTGNVKLKGIIIMGEDDDSHPSEMRLYKNIPQMSFDDTEREPDQTFSLNRDLTGELEYATKISRFSNVYHLSIHISKNFGADTTKVFYIGLRGEWTELRRHEVTICNYEASANPADHRVHQVTPQTHFIS
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Predicted Molecular Mass
31.6 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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