PLA2G2A Protein, Mouse (P.pastoris, His)

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Based on 3 publication(s) in Google Scholar

PLA2G2A Protein, a phospholipase A2 enzyme, efficiently hydrolyzes phosphatidylethanolamines and phosphatidylglycerols, contributing to lipid membrane remodeling and generating lipid mediators crucial for pathogen clearance.PLA2G2A Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived PLA2G2A protein, expressed by P.pastoris , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: P. pastoris
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

PLA2G2A Protein, a phospholipase A2 enzyme, efficiently hydrolyzes phosphatidylethanolamines and phosphatidylglycerols, contributing to lipid membrane remodeling and generating lipid mediators crucial for pathogen clearance.PLA2G2A Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived PLA2G2A protein, expressed by P.pastoris , with C-His labeled tag.

Background

This passage describes the activity and functions of a phospholipase A2 enzyme. Phospholipase A2 is an enzyme that hydrolyzes phospholipids, specifically phosphatidylethanolamines and phosphatidylglycerols, more efficiently than phosphatidylcholines. This enzyme is involved in lipid remodeling of cellular membranes and the generation of lipid mediators that are important in clearing pathogens from the body.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Mouse
  • Source P. pastoris
  • Tag C-His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • PLA2G2A (N22-C146)
      Accession # P31482
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    PLA2G2A; Non-Pancreatic Secretory Phospholipase A2; Prev. PLA2B; Phospholipase A2; Prev. PLA2L; RASF-A; Phosphatidylcholine 2-Acylhydrolase 2A; PLA2S; Phospholipase A2, Membrane Associated; PLAS1; Group IIA Phospholipase A2; SPLA2; GIIC SPLA2; MOM1; Phosp

  • AA Sequence

    NIAQFGEMIRLKTGKRAELSYAFYGCHCGLGGKGSPKDATDRCCVTHDCCYKSLEKSGCGTKLLKYKYSHQGGQITCSANQNSCQKRLCQCDKAAAECFARNKKTYSLKYQFYPNMFCKGKKPKC

  • Predicted Molecular Mass

    15.3 kDa

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 50 mM NaAC, pH 4.0, 5% trehalose, 5% mannitol and 0.01% Tween 80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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