PLK4 Protein, Human
Based on 1 Customer Validation
PLK4 Protein, Human is the recombinant human-derived PLK4, expressed by E. coli , with tag Free labeled tag. ,
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
PLK4 Protein, Human is the recombinant human-derived PLK4, expressed by E. coli , with tag Free labeled tag. ,
PLK4 is a serine/threonine-protein kinase that plays a central role in centriole duplication by triggering procentriole formation on parental centrioles and recruiting biogenesis proteins like SASS6, CPAP, and gamma-tubulin. Overexpression induces centrosome amplification via multiple procentriole generation during S phase. It phosphorylates FBXW5 at Ser-151 to suppress SASS6 ubiquitination and regulates G1/S transition. Dysregulation may contribute to tumorigenesis due to frequent centrosome aberrations in tumors. PLK4 also mediates deuterosome-dependent centriole amplification (producing >100 centrioles) in multiciliated cells and promotes trophoblast differentiation by phosphorylating HAND1. Additional substrates include CDC25C, CHEK2, CEP131 (Ser-78), and PCM1 (Ser-372), the latter being critical for centriolar satellite integrity. by similar: PLK4 is required for STIL recruitment and STIL-driven centriole amplification.
The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
O00444-1 (A2-D275)
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Gene ID10733
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Protein Length
Partial
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Synonyms
SAK; STK18
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AA Sequence
ATCIGEKIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLSNLLLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDVWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSVLDHPFMSRNSSTKSKD
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Predicted Molecular Mass
31.3 kDa
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Molecular Weight
Approximately 30 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 250 mM NaCl.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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