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PPA1 Protein, S. cerevisiae (His)

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The PPA1 protein is an important member of the PPase family and plays an important role in cellular processes, especially dephosphorylation related to phosphate metabolism. PPA1 shares conserved features with related proteins and is involved in phosphatase activity. PPA1 Protein, S. cerevisiae (His) is the recombinant human-derived PPA1 protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Others
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for PPA1 Protein, S. cerevisiae (His)
  • PPA1 Protein, S. cerevisiae (His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The PPA1 protein is an important member of the PPase family and plays an important role in cellular processes, especially dephosphorylation related to phosphate metabolism. PPA1 shares conserved features with related proteins and is involved in phosphatase activity. PPA1 Protein, S. cerevisiae (His) is the recombinant human-derived PPA1 protein, expressed by E. coli , with tag free.

Background

The PPA1 Protein is an integral member of the PPase family, highlighting its significant role in cellular processes. As part of this enzyme family, PPA1 likely shares conserved structural and functional features with related proteins, signifying its involvement in phosphatase activities. The classification within the PPase family underscores its specific designation within the broader context of phosphatases, providing insights into its unique enzymatic functions. The study of PPA1 contributes to our understanding of its role in cellular processes related to dephosphorylation, offering potential applications in therapeutic interventions and a deeper comprehension of its broader impact on cellular processes involved in phosphate metabolism. Further exploration of PPA1's role holds promise for enhancing our knowledge of its contributions to both normal physiology and pathological conditions.

Verified Bioactivity

Measured by its ability to hydrolyze pyrophosphate. The specific activity is 167023 pmol/min/μg, as measured under the described conditions.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Others
  • Source E. coli
  • Tag N-6*His;Tag Free
  • Accession

    A6ZKV8 (M1-V287)

  • Gene ID
    852296  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • PPA1 (M1-V287)
      Accession # A6ZKV8
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    PPA1; Pyrophosphatase (Inorganic) 1; Prev. PP; Epididymis Secretory Sperm Binding Protein Li 66p; Pyrophosphate Phospho-Hydrolase; Diphosphate Phosphohydrolase; IOPPP; Pyrophosphatase (Inorganic); Inorganic Pyrophosphatase; Inorganic Diphosphatase; SID6-8

  • AA Sequence

    MTYTTRQIGAKNTLEYKVYIEKDGKPVSAFHDIPLYADKENNIFNMVVEIPRWTNAKLEITKEETLNPIIQDTKKGKLRFVRNCFPHHGYIHNYGAFPQTWEDPNVSHPETKAVGDNDPIDVLEIGETIAYTGQVKQVKALGIMALLDEGETDWKVIAIDINDPLAPKLNDIEDVEKYFPGLLRATNEWFRIYKIPDGKPENQFAFSGEAKNKKYALDIIKETHDSWKQLIAGKSSDSKGIDLTNVTLPDTPTYSKAASDAIPPASPKADAPIDKSIDKWFFISGSV

  • Predicted Molecular Mass

    33.7 kDa

  • Molecular Weight

    Approximately 34 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 10 mM Tris, 50 mM NaCl, 1 mM DDT, 0.1 mM EDTA, 50% Glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • PPA1 Protein, S. cerevisiae (His) Product Image
    1/16
  • Experimental Validation Results for PPA1 Protein, S. cerevisiae (His)
  • PPA1 Protein, S. cerevisiae (His) Product Image