1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Isomerases (EC 5)
  4. PPIH Protein, Human (His)

PPIH Protein, Human (His)

Cat. No.: HY-P71029
Handling Instructions

The PPIH protein is a peptidyl prolyl cis-trans isomerase (PPIase) that catalyzes the isomerization of prolyl imide peptide bonds and may contribute to protein folding. It plays a crucial role in pre-mRNA splicing and contributes to the formation of the U4/U5/U6 tri-snRNP complex in spliceosome assembly. PPIH Protein, Human (His) is the recombinant human-derived PPIH protein, expressed by E. coli , with N-6*His labeled tag. The total length of PPIH Protein, Human (His) is 177 a.a., with molecular weight of ~24.0 kDa.

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Description

The PPIH protein is a peptidyl prolyl cis-trans isomerase (PPIase) that catalyzes the isomerization of prolyl imide peptide bonds and may contribute to protein folding. It plays a crucial role in pre-mRNA splicing and contributes to the formation of the U4/U5/U6 tri-snRNP complex in spliceosome assembly. PPIH Protein, Human (His) is the recombinant human-derived PPIH protein, expressed by E. coli , with N-6*His labeled tag. The total length of PPIH Protein, Human (His) is 177 a.a., with molecular weight of ~24.0 kDa.

Background

PPIH Protein functions as a peptidyl-prolyl cis-trans isomerase (PPIase), facilitating the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and potentially aiding in protein folding. Beyond its role in protein folding, PPIH actively participates in pre-mRNA splicing, suggesting involvement in the intricate process of spliceosome assembly. Specifically, it may contribute to the formation of the U4/U5/U6 tri-snRNP complex, a fundamental component of the spliceosome. This multifunctional role implies that PPIH may act as a chaperone, highlighting its versatile involvement in both protein folding and the regulation of essential cellular processes related to mRNA splicing. Further exploration is warranted to elucidate the specific molecular mechanisms and cellular contexts through which PPIH exerts its functions in protein homeostasis and pre-mRNA splicing.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

O43447 (M1-M177)

Gene ID
Molecular Construction
N-term
6*His
PPIH (M1-M177)
Accession # O43447
C-term
Synonyms
Peptidyl-Prolyl Cis-Trans Isomerase H; PPIase H; Rotamase H; Small Nuclear Ribonucleoprotein Particle-Specific Cyclophilin H; CypH; U-snRNP-Associated Cyclophilin SnuCyp-20; USA-CYP; PPIH; CYP20; CYPH
AA Sequence

MAVANSSPVNPVVFFDVSIGGQEVGRMKIELFADVVPKTAENFRQFCTGEFRKDGVPIGYKGSTFHRVIKDFMIQGGDFVNGDGTGVASIYRGPFADENFKLRHSAPGLLSMANSGPSTNGCQFFITCSKCDWLDGKHVVFGKIIDGLLVMRKIENVPTGPNNKPKLPVVISQCGEM

Molecular Weight

Approximately 24.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

PPIH Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PPIH Protein, Human (His)
Cat. No.:
HY-P71029
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