PpSQ1_00405 Protein, Pseudomonas putida
The PpSQ1_00405 protein promotes the oxidation of sulfoquinolose to 6-deoxy-6-sulfo-D-glucono-1,5-lactone, showing a significant preference for NAD(+) as the electron acceptor. This enzymatic activity is an important component of the sulfoquinolose (SQ) degradation pathway, allowing P. putida strain SQ1 to utilize SQ as the sole carbon and energy source for its growth. PpSQ1_00405 Protein, Pseudomonas putida is the recombinant PpSQ1_00405 protein, expressed by E. coli , with tag free.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Biological Activity
Description
The PpSQ1_00405 protein promotes the oxidation of sulfoquinolose to 6-deoxy-6-sulfo-D-glucono-1,5-lactone, showing a significant preference for NAD(+) as the electron acceptor. This enzymatic activity is an important component of the sulfoquinolose (SQ) degradation pathway, allowing P. putida strain SQ1 to utilize SQ as the sole carbon and energy source for its growth. PpSQ1_00405 Protein, Pseudomonas putida is the recombinant PpSQ1_00405 protein, expressed by E. coli , with tag free.
Background
PpSQ1_00405 is an enzyme that plays a pivotal role in the degradation pathway of sulfoquinovose (SQ) in Pseudomonas putida SQ1, enabling the bacterium to utilize SQ as its exclusive carbon and energy source for growth. This protein catalyzes the oxidation of sulfoquinovose, converting it into 6-deoxy-6-sulfo-D-glucono-1,5-lactone. Notably, PpSQ1_00405 exhibits a strong preference for NAD(+) as the electron acceptor in this oxidation reaction. The enzyme's ability to efficiently catalyze the conversion of sulfoquinovose underscores its significance in the microbial metabolism of SQ, shedding light on the pathways bacteria employ to utilize this sulfolipid as a nutrient source for sustaining growth and energy production.
Technical Parameters
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Species Others
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Source E. coli
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Tag Tag Free
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Accession
P0DOV5 (N2-Q260)
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Gene ID/
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Molecular Construction
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N-term
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PpSQ1_00405 (N2-Q260)
Accession # P0DOV5 -
C-term
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Protein Length
Full Length
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Synonyms
Sulfoquinovose 1-dehydrogenase; EC:1.1.1.390; SQ dehydrogenase; PpSQ1_00405
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AA Sequence
NRHTDTHYPSLADKVVLISGGASGIGRAFVEAFVAQGSRVAFLDLDAEAGQGLAHALGANSLFLPCDVRDIERLKACVAEVERTWGAVDVLINNAARDDRHALADVSVEYWDERMQTNLRHAFFAAQAVAPGMARRGSGAIINMGSISWMRGRPGMVCYTTAKAALNGMTRTLARELGGQGIRINSLVPGAIRTERQDAMWAADPAGLEAASQAFIDQQMLKFRLDASDCARLALFLASDDSRGCTGQNFVVDAGLSIQ
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)