PpSQ1_00405 Protein, Pseudomonas putida

The PpSQ1_00405 protein promotes the oxidation of sulfoquinolose to 6-deoxy-6-sulfo-D-glucono-1,5-lactone, showing a significant preference for NAD(+) as the electron acceptor. This enzymatic activity is an important component of the sulfoquinolose (SQ) degradation pathway, allowing P. putida strain SQ1 to utilize SQ as the sole carbon and energy source for its growth. PpSQ1_00405 Protein, Pseudomonas putida is the recombinant PpSQ1_00405 protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Others
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

The PpSQ1_00405 protein promotes the oxidation of sulfoquinolose to 6-deoxy-6-sulfo-D-glucono-1,5-lactone, showing a significant preference for NAD(+) as the electron acceptor. This enzymatic activity is an important component of the sulfoquinolose (SQ) degradation pathway, allowing P. putida strain SQ1 to utilize SQ as the sole carbon and energy source for its growth. PpSQ1_00405 Protein, Pseudomonas putida is the recombinant PpSQ1_00405 protein, expressed by E. coli , with tag free.

Background

PpSQ1_00405 is an enzyme that plays a pivotal role in the degradation pathway of sulfoquinovose (SQ) in Pseudomonas putida SQ1, enabling the bacterium to utilize SQ as its exclusive carbon and energy source for growth. This protein catalyzes the oxidation of sulfoquinovose, converting it into 6-deoxy-6-sulfo-D-glucono-1,5-lactone. Notably, PpSQ1_00405 exhibits a strong preference for NAD(+) as the electron acceptor in this oxidation reaction. The enzyme's ability to efficiently catalyze the conversion of sulfoquinovose underscores its significance in the microbial metabolism of SQ, shedding light on the pathways bacteria employ to utilize this sulfolipid as a nutrient source for sustaining growth and energy production.

Technical Parameters

  • Species Others
  • Source E. coli
  • Tag Tag Free
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • PpSQ1_00405 (N2-Q260)
      Accession # P0DOV5
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    Sulfoquinovose 1-dehydrogenase; EC:1.1.1.390; SQ dehydrogenase; PpSQ1_00405

  • AA Sequence

    NRHTDTHYPSLADKVVLISGGASGIGRAFVEAFVAQGSRVAFLDLDAEAGQGLAHALGANSLFLPCDVRDIERLKACVAEVERTWGAVDVLINNAARDDRHALADVSVEYWDERMQTNLRHAFFAAQAVAPGMARRGSGAIINMGSISWMRGRPGMVCYTTAKAALNGMTRTLARELGGQGIRINSLVPGAIRTERQDAMWAADPAGLEAASQAFIDQQMLKFRLDASDCARLALFLASDDSRGCTGQNFVVDAGLSIQ

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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