PRC1 Protein, Human (sf9, His)

PRC1 is a key regulator of cytokinesis, coordinating midzone formation and successful cell division by cross-linking microtubules and recruiting essential proteins such as KIF14, PLK1 and ECT2. Its effects extend to tumorigenesis, promoting bladder cancer cell proliferation and inhibiting apoptosis. PRC1 Protein, Human (sf9, His) is the recombinant human-derived PRC1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of PRC1 Protein, Human (sf9, His) is 620 a.a., with molecular weight of ~75 kDa.

PRC1, a pivotal regulator of cytokinesis, plays a crucial role in orchestrating the spatiotemporal formation of the midzone and ensuring successful cytokinesis by cross-linking antiparallel microtubules at an average distance of 35 nM. Its indispensability extends to the recruitment of key proteins such as KIF14, PLK1, and ECT2 to the central spindle and midbody, essential steps for proper cell division. In addition to its fundamental role in cellular processes, PRC1 exhibits oncogenic properties, promoting proliferation, inhibiting apoptosis, and contributing to the carcinogenic progression of bladder cancer cells. As a homodimer, PRC1 interacts with various proteins, including RACGAP1, CENPE, KIF4A, KIF23, and PLK1, forming a complex network of molecular interactions crucial for its multifaceted functions during mitosis. The intricate interplay of PRC1 with these proteins underscores its significance in the intricate machinery governing cell division and highlights its potential as a target for therapeutic interventions in cancer.

Human

Sf9 insect cells

N-His

O43663 (M1-S620)

9055  [NCBI]

Approximately 75 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 20% Glycerol, 3 mM DTT. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping with dry ice.