Prok2 Protein, Mouse (His-B2M)

Prok2 Protein, Mouse (His-B2M) is the recombinant mouse-derived Prok2 protein, expressed by E. coli, with N-B2M & N-6*His tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

Prok2 Protein, Mouse (His-B2M) is the recombinant mouse-derived Prok2 protein, expressed by E. coli, with N-B2M & N-6*His tag.

Background

Prok2 may function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. It could also act locally within the SCN to synchronize output. Additionally, Prok2 potently contracts gastrointestinal (GI) smooth muscle (by similarity).

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag N-B2M;N-6*His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • 6*His-B2M
    • Prok2 (A27-K128)
      Accession # Q9QXU7
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    PROK2; MIT1; Prokineticin 2; KAL4; PK2; Prokineticin-2; BV8; HH4; Protein Bv8 Homolog

  • AA Sequence

    AVITGACDKDSQCGGGMCCAVSIWVKSIRICTPMGQVGDSCHPLTRKSHVANGRQERRRAKRRKRKKEVPFWGRRMHHTCPCLPGLACLRTSFNRFICLARK

  • Predicted Molecular Mass

    24.02 kDa

  • Molecular Weight

    Approximately 26 kDa, based on SDS-PAGE under reducing conditions.

  • Structure/Form

    Monomer

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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