PRPF19 Protein, Human (sf9, GST)
PRPF19 Protein, Human (sf9, GST) is the recombinant human-derived PRPF19, expressed by Sf9 insect cells , with GST labeled tag. ,
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
PRPF19 Protein, Human (sf9, GST) is the recombinant human-derived PRPF19, expressed by Sf9 insect cells , with GST labeled tag. ,
Background
PRPF19 is a ubiquitin-protein ligase that serves as a core component of several complexes primarily involved in pre-mRNA splicing and DNA repair. As part of the spliceosome, it facilitates assembly, remodeling, and activity, including mediating 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3 to stabilize the U4/U5/U6 tri-snRNP complex. Recruited to RNA polymerase II CTD, it may couple transcription with splicing machinery. In DNA damage response (DDR), PRPF19 (within the PRP19-CDC5L complex) ubiquitinates RPA1/RPA2 at damage sites to activate the ATR pathway. It also contributes to DNA double-strand break (DSB) repair by recruiting SETMAR and potentially participates in DNA interstrand cross-link (ICL) repair via the PSO4 complex. PRPF19 may additionally mediate 'Lys-48'-linked polyubiquitination for proteasomal degradation. By similarity, it might play roles in lipid droplet biogenesis and neural differentiation through spliceosomal functions.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag GST
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Accession
Q9UMS4 (S2-L504)
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Gene ID27339
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Protein Length
Full Length of Mature Protein
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Synonyms
PRPF19; RING-Type E3 Ubiquitin Transferase PRP19; Prev. PRP19; Senescence Evasion Factor; NMP200; PRP19/PSO4 Homolog; SNEV; Psoralen 4; Pre-MRNA-Processing Factor 19; PRP19/PSO4 Pre-MRNA Processing Factor 19 Homolog (S. Cerevisiae); UBOX4; PRP19/PSO4 Pre-
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AA Sequence
SLICSISNEVPEHPCVSPVSNHVYERRLIEKYIAENGTDPINNQPLSEEQLIDIKVAHPIRPKPPSATSIPAILKALQDEWDAVMLHSFTLRQQLQTTRQELSHALYQHDAACRVIARLTKEVTAAREALATLKPQAGLIVPQAVPSSQPSVVGAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVKPEELSKYRQVASHVGLHSASIPGILALDLCPSDTNKILTGGADKNVVVFDKSSEQILATLKGHTKKVTSVVFHPSQDLVFSASPDATIRIWSVPNASCVQVVRAHESAVTGLSLHATGDYLLSSSDDQYWAFSDIQTGRVLTKVTDETSGCSLTCAQFHPDGLIFGTGTMDSQIKIWDLKERTNVANFPGHSGPITSIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQLDNNFEVKSLIFDQSGTYLALGGTDVQIYICKQWTEILHFTEHSGLTTGVAFGHHAKFIASTGMDRSLKFYSL
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Molecular Weight
Approximately 74 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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