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PTK7 Protein, Rabbit (HEK293, His)

Customer Review

Based on 1 Customer Validation

The PTK7 protein is an inactive tyrosine kinase that plays a key role in both non-canonical and canonical Wnt signaling pathways. Effects include cell adhesion, migration, polarity, proliferation, actin cytoskeleton reorganization, and apoptosis. PTK7 Protein, Rabbit (HEK293, His) is the recombinant Rabbit-derived PTK7 protein, expressed by HEK293 , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rabbit
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for PTK7 Protein, Rabbit (HEK293, His)
  • Experimental Validation Results for PTK7 Protein, Rabbit (HEK293, His)
  • PTK7 Protein, Rabbit (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

The PTK7 protein is an inactive tyrosine kinase that plays a key role in both non-canonical and canonical Wnt signaling pathways. Effects include cell adhesion, migration, polarity, proliferation, actin cytoskeleton reorganization, and apoptosis. PTK7 Protein, Rabbit (HEK293, His) is the recombinant Rabbit-derived PTK7 protein, expressed by HEK293 , with C-10*His labeled tag.

Background

The PTK7 protein, an inactive tyrosine kinase, plays a significant role in the Wnt signaling pathway, being a component of both the non-canonical (Wnt/planar cell polarity signaling) and canonical Wnt signaling pathways. Its involvement spans diverse cellular processes, including cell adhesion, migration, polarity, proliferation, actin cytoskeleton reorganization, and apoptosis. PTK7 also contributes to critical developmental events, such as embryogenesis, epithelial tissue organization, and angiogenesis. Notably, it interacts with CTNNB1, underlining its participation in Wnt pathway regulation and its impact on cellular functions with broader implications in both normal and pathological contexts.

Verified Bioactivity

1.This product does not contain protein kinase domain.
2.Immobilized PTK-7 at 2 μg/mL (100 μL/well) can bind Biotinylated Wnt3a protein. The ED50 for this effect is 8.625 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized PTK-7 at 2 μg/mL (100 μL/well) can bind Biotinylated Wnt3a protein. The ED50 for this effect is 8.625 μg/mL.

Technical Parameters

  • Species Rabbit
  • Source HEK293
  • Tag C-10*His
  • Accession

    G1SIJ6-1 (A31-T612)

  • Gene ID
    /
  • Molecular Construction
    • N-term
    • PTK7 (A31-T612)
      Accession # G1SIJ6-1
    • 10*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    PTK7; PTK7 Protein Tyrosine Kinase 7; Protein Tyrosine Kinase 7 (Inactive); Tyrosine-Protein Kinase-Like 7; CCK4; CCK-4; Colon Carcinoma Kinase 4; Protein Tyrosine Kinase 7; Inactive Tyrosine-Protein Kinase 7; Protein-Tyrosine Kinase 7; Pseudo Tyrosine Ki

  • AA Sequence

    AIVFIKEPSSQDALQGRRALLRCEVEAPAPVHVYWLLNGAPVQDTERRFVQDTERRFPQVSSLSFTAVDRLQDSGAFQCVARDNVTGEEARSANASFNIKWIEGGPVVLKQPASEAEIQPQTQVTLRCHVDGHPRPTYQWFRDGTPLSDGQSNHTVSSKERNLTLRPATPEHSGVYSCCAHNAFGQACSSQNFTLNIAEIEDMPPFEPRVFTAGSEERVACLPPHGLPEPSVWWEQAGVRLPTHGRVYQKGHELVFADTAESDAGVYTCHAANLAGQRRQDVNITVATVPTWLEKPQDSQLEEGRPGYLHCLTQATPKPTVVWYRNQMLISEDSRFEVSKNGTLRINSVEVYDGTWYRCVSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTIKWIRADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNGPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLRCEAQGDPKPLIQWKGKDRILDPSKLGPRMHIFQNGSLVIHDVAPEDSGRYTCIAGNSCNIKHTEAPLYVVDKPTPEESEGPGSPPPYKMIQT

  • Molecular Weight

    Approximately 100-115 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized PTK-7 at 2 μg/mL (100 μL/well) can bind Biotinylated Wnt3a protein. The ED50 for this effect is 8.625 μg/mL.

  • PTK7 Protein, Rabbit (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for PTK7 Protein, Rabbit (HEK293, His)
  • Experimental Validation Results for PTK7 Protein, Rabbit (HEK293, His)
  • PTK7 Protein, Rabbit (HEK293, His) Product Image